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Researchers studying protein localization and trafficking now have a convenient tool: the MinuteTM Water-soluble and Water-Insoluble Protein Fractionation Kit for cells/tissues. This unique product allows for the rapid and efficient separation of these two key fractions from cells/tissues, microorganisms and plant tissues, providing enriched and easily detectable protein samples for downstream applications like western blotting, ELISA and immunoprecipitation. Water soluble fraction contains mainly cytosolic proteins and water-insoluble fraction contains mainly insoluble membranous structures such as plasma membrane, nuclear membrane and organelles. The kit offers researchers a novel and simple approach for understanding protein function and movement within the cellular landscape. If a protein target is known to be localized in water-soluble or water-insoluble fraction, the kit will facilitate detection, isolation and purification of the target protein by enrich corresponding fractions.

Fig. 1. Protein profiles of water-insoluble (WI) and water-soluble (WS) fractions from HEK293 cells, yeast (S. cerevisiae) and plant (Broccoli leaf). Proteins were  separated in 10% SDS-PAGE and stained with Coomassie blue.

Fig. 2. Western blotting of fractionated frozen mouse liver. Lanes 1, 2 and 3 are total liver lysate,  water-insoluble and water-soluable fraction, respectively. A. Ponceau red stained blot. B. Western blot with anti-Na/K-ATPase (plasma membrane marker), Lamin B1 (nuclear envelope marker) and GAPDH (cytosolic marker).