Products

AHARIBO Translatome RNASeq

Immagina Biotechnology, the Ribosome company, released today the AHARIBO Translatome RNAseq

system, the first commercially available complete system to analyse gene expression at the ribosome level

(Translatome) based upon the detection of full-length mRNAs from actively translating ribosomes.

The new AHARIBO Translatome RNA-seq system is an all-in-one kit dedicated to analyze actively translated

full-length RNAs by capturing active ribosomes, isolate their RNA content and convey the RNAs into an NGS

library.

Features:

Easy all-in-one protocol

• enables to generate ready to sequence translatome libraries starting from RNA purified from a wide range of

  cell lines after AHARIBO capture of active ribosomes

Streamlined generation of robust Illumina compatible libraries

• Within 7.5 hours

• Starting from as little as 50.000 (corresponding to at least 1 ug of total RNA)

• Starting from as little as 50.000 (corresponding to at least 1 ug of total RNA)

• Ribosomal RNA depletion module included

• Reproducible sequencing data

• Libraries contain UMIs and dual indexes for higher accurate sequencing

• Libraries compatible with single-read and paired-end sequencing

Fig. 1. Overview of the AHARIBO_TRANSLATOME_RNAseq kit workflow

High Reproducibility

AHARIBO Translatome RNAseq exhibits outstanding reproducibility and correlation across replicates.

Applications;

Low input requirements, an easy work flow without the need for expensive laboratory equipment such as an

ultracentrifuge and fractionators combined with a superior RNAseq library preparation system makes the

AHARIBO Translatome RNAseq system a versatile tool for many application areas.

The AHARIBO Translatome RNAseq system is suitable for a wide range of cell culture-based applications

such as:

• measuring global translation rates

• studying alternative splice variants,

• translational events in poorly annotated ncRNAs

By popular demand, finally available as complete solution from sample prep to library prep. The kit includes

• A module for isolation of active ribosomes and full-length translated RNAs

• A module for rRNA depletion

• An Illumina-compatible NGS library preparation with UDIs plate included

• A module for optimal PCR-cycle number determination by qPCR

Kit contents;

 Protocol (PDF)