Products

Protein

Gelsolin TL40 (N-terminal cytoplasmic gelsolin)

Origin

Mouse, recombinant

Molecular mass

40kDa

Product description

Recombinant  mouse TL40 is the N-terminal part of mouse gelsolin and comprises

the homologous gelsolin subdomains S1-S3 (aa 1-361, molacular mass: ~40kD).

TL40 is a Ca++-independent part of the gelsolin molecule. It is used in all assays

with gelsolin, where Ca++ has to be avoided. The Ca++ independence is a natral

feature of N-terminal gelsolin protein.

TL40 is preferentially used in F-actin fragmentation assays in the presence of EGTA.

Actin interaction

Ca++-insensitive F-actin severing.

Form

Lyophilized, ready-to-use.

Quantity

1 x 50 µg

Buffer

Lyophilized TL40 (50µg) contains 20mM imidazole pH 7.2, 50mM KCl, 1.0mM EGTA

and 0.1mM DTT and 0.5% disaccharides,  when reconstituted with 50µl ultrapure

water to obtain a 1.0 mg/ml solution.

Purity & Activity

Purity: >98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Fragmentation activity: The fragmentation activity of TL40 was determined by

Ostwald viscometry at 25°C. The specific viscosity of F-actin (1mg/ml) is reduced

by <42% after addition of TL40 at a molar ratio of 1 : 50 (TL40 : A) within less than

2min.

For this purpose the TL40 stock solution was used as described below containing

1% disaccharides. F-actin (100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP) was

mixed with TL40 and instantly measured within a time course of 0-30min by

Ostwald viscometry.

Purification notes

GPC, IEXC.

Protein concentration

Determined at A280 0.1209 = 1mg/ml  

Storage instructions

TL40 is stored at –70°C upon arrival will be stable in performance for at least 6

months from the date of purchase. The solubilized protein is kept on ice and should

be used within 5 days. Storage in glycerol at -20°C results in a significant decrase

of the activity, and has to determined individually.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.