Products

Cellular Fractionation, FOCUS™ Glycoprotein

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl

and α-D glycosyl proteins.

FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that

contain lectin (Concanavalin A) bound resin with the capacity to bind and immobilize ~20mg glycoproteins.

Column bound glycoproteins are eluted with a set of three rapid elution buffers. The proprietary serial elution

allows for faster elution compared to other glycoprotein purification systems.

The eluted proteins are suitable for 2D electrophoresis and isoelectric focusing.

Features;

•  Spin column protocol

•  Uses a high capacity lectin binding resin (10-16mg/ml resin)

•  Elution of glycoproteins within 90 minutes with a set of three rapid elution buffers

Application;

• Fractionation and enrichment of glycoproteins suitable for 2D eletrophoresis and other applications

 Instruction sheet (PDF)