|
Serotonin ELISA kit
This Serotonin (5-HT) ELISA kit enables the ultra-sensitive measurement of 5-hydroxytryptamine in any
biological sample. Intended for research use only, the assay features a lower limit of detection at 5 pg/ml
and a minimal sample volume as low as 1µl.
Specifications
|
Format;
|
96-well plate (40 samples in duplicate)
|
|
Sample;
|
All biological samples
|
|
Sample volume;
|
1 ~ 100 µl
|
|
Species-reactivity;
|
React with all species
|
|
Standard range;
|
0 / 0.015 ~ 2.5 ng/ml
|
|
Sensitivity;
|
5 pg/ml
|
|
Specificity;
|
No significant cross-reactivity was observed with Serotonin analogs such
as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine,
Histidine, Tyramine and 5-Hydroxytryptophan.
|
|
Assay time;
|
30 min sample preparation, ELISA overnight
|
|
Storage;
|
Store at 2 ~ 8°C for to 6 months.
|
Instruction sheet (PDF)
References;
1. Valiyaveettil M et al. Contribution of systemic factors in the pathophysiology of repeated blast-induced neurotrauma. Neurosci Lett. 2013 Feb 28;539:1-6.
2. Damen R et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One. 2013 Apr 26;8(4):e62607.
3. Kaushal N et al. Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol. 2013 Aug;23(8):960-71.
4. Rokstad AM et al. Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies. Cancer Sci. 2012 Jul;103(7):1230-7.
5. Chin A et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol. 2012 Feb 1;302(3):G397-405.
6. van den Buuse M. Exploring the role of 5-HT1A receptors in the regulation of prepulse inhibition in mice: implications for cross-species comparisons. ACS Chem Neurosci. 2013 Jan 16;4(1):149-60.
|