Products

CytoScan™ - fluoro / SRB / LDH Cytotoxicity Assay

CytoScan™ - fluoro Cytotoxicity Assay

The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when

cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured

with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluorescent compound

(resazurin) to a fluorescent compound (resorufin), which is measured using a fluorometer. The assay

detectseven low level damage to cell membrane not detected with other methods.

The CytoScan™-fluoro kit does not damage healthy cells and the assay can be performed directly in the cell

culture wells containing a mixed population of viable and damaged cells. The kit is supplied with substrate

mix, assay buffer, and stop solution.

Features

• Quantitatively measures LDH release

• Fluorometric assay

• Assays performed directly in cell culture wells

Applications

• For the detection of cell toxicity, death, viability or proliferation.

• Ideal for high throughout in cell culture wells

References

• Mohan, S. et al (2014) NeuroToxicology. DOI: 10.1016/j.neuro.2014.10.012

• Mohan, S. et al (2013) Front. Mol. Neurosci. 6:31

Instruction sheet (PDF)

CytoScan™ - SRB Cytotoxicity Assay

CytoScan™-SRB Cytotoxicity Assay is an accurate and reproducible assay based upon the quantitative

staining of cellular proteins by sulforhodamine B (SRB).

This assay has been used for high-throughput drug screening at the National Cancer Institute (NCI) (1).

Sulforhodamine B is an anionic aminoxanthene dye that forms an electrostatic complex with the basic amino

acid residues of proteins under moderately acid conditions, which provides a sensitive linear response. The

color development is rapid and stable and is readily measured at absorbances between 560 and 580nm. The

kit components are sufficient for performing up to 1000 assays.

Features

• Measures total biomass by staining cellular proteins.

• Linear response.

• Simple, accurate and reproducible assay.

Applications

• For the detection of cell toxicity, death, viability, or proliferation.

• Ideal for high throughout screening.

References

• Zhou, W.P. et al (2015) Indian J Pharm Sci. 77:449

• Cross, C. et aql (2015) Biomed Res Int 25:7090

• Tolstikov, V. et al (2014) PLOS. DOI: 10.1371/journal.pone.0114019

• Xiao, Y. et al (2013) Neoplasia 15:1151

• Xiao, Y. et al (2013) Neoplasia 15:1137

• Boyerinas, B et al (2012) IJC. 130:1787

• Zhao, Y. et al (2012) ACS Nano. 6:8684

• Haque, F. et al (2012) Nanotoday. 7:245

Instruction sheet (pdf)

CytoScan™ - LDH Cytotoxicity Assay

The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an

alternative to 51Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple

evaluation, characteristic of radioisotope release assays, with the convenience of accuracy and avoidance of

radioactivity. The CytoScan™ LDH Cytotoxicity Assay Kit can be used with different cell typesfor assaying

cell mediated cytotoxicity as well as cytotoxicity mediated by chemicals and other test compounds.

The assay quantitatively measures the stable, cytosolic, lactate dehydrogenase (LDH) enzyme, which is

released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results

in the conversion of a tetrazolium salt (iodonitrotetrazolium (INT)) into a red color formazan by diaphorase.

The LDH activity is determined as NADH oxidation or INT reduction over a defined time period.

The resulting formazan absorbs maximally at 492nm and can be measured quantitatively at 490nm.

Features

• Quantitatively measures LDH release

• Colorimetric assay

• Ideal for high throughout screening.

Applications

• For the detection of cell toxicity, death, viability, or proliferation.

• For cell free supernatants from cells in culture (adherent or suspension)

References

• Blackler, R.W. et al (2015) Am J Physiol. DOI: 10.1152/ajpgi.00066.2015

• Benien, P. et al (2015) J. Liposome. Res. DOI: 10.3109/08982104.2015.1022557

• Li, W. H. et al (2015) Dermatol Ther. DOI: 10.1007/s13555-015-0072-7

Instruction sheet (pdf)

Catalog No.

Product Name

Size

786-211

                CytoScan™ - fluoro Cytotoxicity Assay

500 assays

786-213

   CytoScan™ - SRB Cytotoxicity Assay

1000 assays

786-210

                   CytoScan™ - LDH Cytotoxicity Assay

1000 assays