Products

AlbuSorb™ PLUS

Albumin + IgG depletion from serum or plasma

•  >400 μg total serum protein mass (> 85% Albumin, >85% IgG depleted) from 25 μl serum prep
•  Affinity-type equivalence, virtually no cross-reactivity with other proteins
•  Disposable, no column regeneration or cross-contamination
•  Combines unique bead technology, not based on Blue-dye affinity, with optimized Protein A
•  Mild conditions maintains structural integrity and simple transfer to secondary analysis
•  Suitable for immunoassay, Western blot, 1 & 2D Electrophoresis, enzyme assay, LC-MS
•  Tested species include human, sheep, bovine, rabbit, mouse, rat

Poly-electrolytes are polymers with repeating units of stationary charges. AlbuSorb™ comes from a class of

solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an

empirically derived chemistry combining elements of polymer composition, cross-linking architecture and

charge properties. AlbuSorb™ combines with an optimized immobilized Protein A to create AlbuSorb™PLUS.

Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-

contamination. AlbuSorb™ PLUS is supplied as a powder. Simply weigh, centrifuge and/or filter, and recover

the {albumin + Immunoglobulin} - depleted serum in the supernatant. 

Fig. 1. Gel Image: SDS-PAGE non-reduced, Criterion™ Tris.HCl (Bio-Rad) 4-15%

1. Human Serum Control (25 μl Serum + 250 μl Buffer)

2. AlbuSorb™ PLUS Flow-Through Analysis by gel estimation & LC-MS Spectral Counts

   Albumin <10%, 85+%+ removal IG annotated <10%, 85+%+ removal