Products

Trans-Hi™, In vitro DNA Transfection Reagent  

Features

• Excellent transfection for single DNA transfection or multi DNA co-transfection

• Rapid onset and long-lasting transfection

• Suitable for very long DNAs

• Simple and easy to use transfection procedure

• Robust and reproducible transfection

• High level of recombinant protein production

• Very cost efficient

• Long shelf-life: 12 months

Specifications:

Fig. 1. Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine? 2000 in

HEK293T cells  with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and

0.5µg DNA per well used for both products in 24 well plate 30 hours post transfection.

Fig. 2. Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal

tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plate 30

hours post transfection, 0.5ug DNA per well).

Table 1. Broad Transfection Spectrum for Mammalian Cell Types

Protocols

Trans-Hi™ General Protocol For In Vitro Transfection in Mammalian Cells

Trans-Hi™ Protocol for Transfecting 293 and CHO Suspension Cells

Trans-Hi™ Protocol for Generation of rAAV

Trans-Hi™ Technical Tips For Good In Vitro Transfection