Products

Exo-FACS, Ready to use kit for exosome FACS analysis

Ready to use kit which allows exosome isolation from biofluids or cell media and Fluorescence-Activated

Cell Sorting (FACS) analysis of exosome markers

Exo-FACS, Exosome isolation and FACS analysis of exosome markers

Exo-FACS allows exosome isolation from biofluids or cell culture media and FACS analysis of exosome

markers. The kit consists of EXO-Prep reagent, for exosome isolation, 4 μm beads used for the overall

capture of pre-isolated exosomes, lyophilized exosomes from cell culture supernatants or human biological

fluids, as positive control. The characterization of exosomal proteins (membrane expressed or internal) is

subsequently performed using appropriate detection antibodies against exosome associated antigens.

Features

• Ready to use

• No initial exosome purification required

• Lyophilized Exosome Standards for positive control included

• User friendly and suitable for multiple marker analyses

Applications

• Exosome isolation and exosome marker characterization via FACS

• Exosome comprehensive profiling

Exo-FACS is an useful tool for exosome protein profiling by using FACS technique

Exo-FACS was used for a protein marker profile in exosomes derived from different sources. Exosome

binding on FACS-beads was performed by incubation at 4°C over night. Exosome-bead complex is ready to

be labeled with fluorophore-conjugated antibodies for specific exosome markers. In figure 1 is shown a

profile of expression of three different exosome markers in exosomes purified from Melanoma (MM1),

Neuroblastoma (SH) and Glioblastoma (U87) cell supernatants.

Fig. 1.  FACS profiling of exosomal markers CD9, CD63 and TM9SF4 in purified exosomes from MM1, SH-

SY5Y and U87 cell lines.

Kit components

HBM offers different Exo-FACS kits for staining of exosomal markers from human biofluids (plasma, urine,

serum, saliva) and from cell culture supernatants. Exo-FACS contains reagents for 20 reactions (lyophilized

exosomes, beads, antibodies and buffers). Primary antibody included in the kit is against a common

exosomal marker (CD9 or CD63) and can be used as a positive control for protein profiling via FACS analysis.

Ordering informations

- Exo-FACS ready to use kit for analysis of exosomal marker from human biofluids

- Exo-FACS ready to use kit for FACS analysis from cell culture media

* Exosome from a wide list of different cell lines can be purified on request. Cell line list is available in

  section 1 of HBM catalog