Premade Human Tissue Northern Blot
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Premade Human Tissue Northern Blot
Human tissue Northern blots are made of high quality total RNA samples which are subjected to rigorous quality control
procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon
membranes.
Applications ;
- Blots can be used with either radioactive or nonisotopic-labeled probes to:
- Quickly assess gene expression across human tissues.
- Determine size and relative abundance of transcripts in different tissues.
- Identify alternative splice forms.
Benefits:
- Ready-to-hybridize Northern blots of total RNA of tissues that are difficult to obtain because of size, high RNase content, size, and anatomical complexity which requires knowledge, experience, and technical skill to exactly dissect the area of interest freely of surrounding tissues.
- Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
- High standards of quality control
- Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to the labor and cost of laboratory materials, supplies and animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
- Reusable for several times, just strip probe and re-hybridize with new one.
Quality control: Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is
carefully monitored to ensure the superior quality and performance.
- Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
- The efficiency of transfer is checked by staining gels with reversible staining .
- The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated total RNA samples (20mg each) are fractionated though large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Each blot
contains 15 samples. Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4°C in
the sealed bags.
|
Lane |
Tissue |
|
RNA Marker |
RNA Marker |
|
1 |
Brain |
|
2 |
Stomach |
|
3 |
Intestine |
|
4 |
Colon |
|
5 |
Liver |
|
6 |
Lung |
|
7 |
Kindney |
|
8 |
Heart |
|
9 |
Ovary |
|
10 |
Skeletal muscle |
|
11 |
Spleen |
|
12 |
Testis |
|
13 |
Tonsil |
|
14 |
Placenta |
|
15 |
Pancreas |
Ordering information ;
1. HN-MT-1 Human Tissue Northern Blot
▣ 관련 페이지 ; Zyagen
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