Products

MicroMolar Primary Amine Assay Kit

The MicroMolar Primary Amine Assay Kit is designed for measurement of primary amines or ammonia or

their salts at micromolar concentrations.  Amino acids, peptides and many drug molecules contain primary

amine groups can be quantified using this kit.  Protonated primary amines or ammonium salts release the

free primary amine or ammonia in the kit assay buffer.  Primary amines or ammonia interact with the assay

reagent PAA dye to form fluorescent products with excitation at 390 nm and emission at 470 nm.   The kit can

be generally used for measurements of micromolar concentrations of primary amines or ammonia or their

salts in biological samples, biochemical reactions, pharmaceutical products and environmental water

samples. The assay is not compatible with amine-based buffers such as Tris buffer or buffers that contain

thiol-based reducing reagents such as DTT.

The MicroMolar Primary Amine Assay Kit (Catalog number PAA100K) includes 0.5 ml of 10 x PPA dye, 5.5 ml

of Assay buffer, 5 ml of reagent A and 0.05 ml of 1 mM methylamine-HCl.  It is for measurement of 100

samples using 96-well plates.  Cuvettes may also be used for measurements.