Products

DTT assay kit

DTT (Dithiothreitol) is a common reducing agent in biochemistry.  Removal of DTT is required for Cys-based

protein labeling and for disulfide bond formation in proteins.  The MicroMolar DTT Assay Kit (Catalog number

DTT200) is designed for measurement of micromolar concentrations of DTT.

The assay is based on increase of fluorescence at 535 nm of the dye C55 in the presence of DTT.  The

assay kit can be used for measurements DTT concentrations in biological samples, biochemical reactions

and environmental water samples. The assay is compatible with HEPES buffer, low concentrations of non-

ionic detergent (<0.01%), MgCl2 (< 5 mM), CaCl2 (<5 mM), Tris-HCl (<10 mM), EDTA (< 1 mM) and

phosphate (< 1 mM). It is not compatible with thiol compounds such as 2-mercaptoethanol or cysteine.

The MicroMolar DTT Assay Kit (catalog number DTT200) provides the reagent for measurement of 200

samples using 96-well plates.  Cuvettes may also be used for measurements.