Products

Human tissue Northern blots are made of high quality total RNA samples extracted from human fresh tissues.

Each RNA sample is subjected to rigorous quality control procedures before fractionation through large

denatured agarose gels and blotting onto positively charged nylon membranes. Blots can be used with either

radioactive or nonisotopic-labeled probes to:

• Quickly assess gene expression across human tissues.
• Determine size and relative abundance of transcripts in different tissues.
• Identify alternative splice forms.

Benefits:

• Ready-to-hybridize Northern blots of total RNA of  tissues that are difficult  to obtain because of size,
• high RNase content, size, and anatomical complexity which requires knowledge, experience, and
• technical skill to exactly dissect the area of interest freely of surrounding tissues.
• Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and
• homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
• High standards of quality control
• Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory.
• Think to the labor and cost of laboratory materials, supplies and  animals (purchasing, transportation,
• housing, disposable of carcasses and waste materials).  
• Reusable for several times, just strip probe and re-hybridize with new one.

Quality control: Every step of preparation of blots, from harvesting tissues and extraction of RNA to the

blotting, is carefully monitored to ensure the superior quality and performance.

• Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity  of RNA are tested by denaturing agarose gel electrophoresis.
• The efficiency of transfer is checked by staining gels with reversible staining .
• The integrity of the blotted RNA is tested by beta-actin specific probe.

Description:The freshly isolated total RNA samples (20mg each) are fractionated though large

formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and

immobilized by UV light. Each blot contains 15 samples. Blots are shipped in a sealed bag at room

temperature and are good for 1 year if stored at 4^oC in the sealed bags.

Catalog No.

Product Name

Size

HN-MT-1

Human Tissue Premade Northern Blot

1 Blot

HN-CUS-1

Human Tissue Custom Made NB-1

1 Blot

HN-CUS-2

Human Tissue Custom Made NB-2

1 Blot