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Aurion Colloidal gold based protein Stain

Col-Aurion is a colloidal gold particle based total protein stain, developed for the sensitive staining of

electrophoretically separated protein bands on nitrocellulose or PVDF™ blotting membranes. Colloidal gold

particles accumulate at the site of the protein bands on the membrane, generating an intense dark red

staining pattern. The total protein stain assists in assessing immunoblotting results and to evaluatethe

effectivity of the blotting procedure.

Introduction

The negative surface charge of colloidal gold particles is responsible for their high affinity for positively

charged macromolecules. This characteristic was the basis for the development of a total protein stain

based on colloidal gold.

Product Description

Col-Aurion is a total protein stain consisting of a solution of coated gold particles with an average particle

diameter of 15 nm. The staining principle is based on the electrostatic binding of negatively charged gold

particles to proteins with a positive charge present at low pH.

A unique feature of Col-Aurion is the use of BSA-c™ to shield off the surface of the gold particles.

Destabilization of gold particles that might occur as a result of interaction with detached protein is thus

prevented. The strong negative charge of BSA-c™ gives an additional increase in sensitivity of the stain.

Col-Aurion total protein stain has a pH of 3.2 and is ready to use. For removal of surplus of weakly bound

protein from the membrane, a 10 ml vial of Tween-20™ is included.

Features

• Ready-to-use solution

• Of BSA-c™ stabilized colloidal gold particles

• For total protein staining on membranes

• With high sensitivity, no overstaining Intense and permanent signal

• Straightforward method Economical use.

Application Instructions

Detailed information is provided in the package insert.Col-Aurion is a sensitive total protein stain for bio

assays, especially nitrocellulose and PVDF Western blots and dot-blots. The protein binding substratum has

to carry a negative charge.

Storage

Col-Aurion has a guaranteed shelf life of 18 months from the date of quality control analysis.

The products should be stored at 4-8°C. Do not freeze.

Instruction sheet (PDF)

Ordering informations   

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