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Conventional Immuno Gold reagents I

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Conventional Immuno Gold reagents I

Conventional Immunogold Reagents are available in four size classes. The monodisperse size population

makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents

are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is

abundant and the accessibility of the antigen is relatively good.

Introduction

The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary

immunogold reagents based on particles that can be observed without enhancement. These conjugates are

suited for single and multiple labeling in electron microscopy, when the number of antigens available for

binding is such that a relevant signal can be obtained.

The AURION Conventional Immunogold Reagents are built around colloidal gold particles with sizes of 6, 10,

15 or 25 nm. The particle population is monodisperse and thus shows minimal size variation and overlap.

Typically, the coefficient of variance for the 6 and 25 nm particle size conjugates is less than 12%, whereas

the 10 and 15 nm size conjugates show less then 10% variation.

The table below lists a few physical characteristics of gold conjugates.

Particle diameter	#Au atoms	MWt. (daltons)	#/particles / ml	#Ab / (particle)
6 nm	6500	1.3·10^6	2.4·10^13	1-2
10 nm	30·10^3	6·10^6	5·10^12	7-12
15 nm	100·10^3	20·10^6	1.5·10^12	25-40
25 nm	470·10^3	92·10^6	3.3·10^11	115-180

Product Description

AURION Conventional Immunogold Reagents are tailored to contain 10-20 µg of specific protein/ml. The

reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3 at an OD520nm of 1.0 to

warrant prolonged cluster free storage. The activity of each lot is determined using a dot-spot test system as

described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353. Actual lot specifications (size,variation

and expiry date) are indicated on the accompanying package insert.

Regular package: for the labeling of 1000-2000 grids

Small package: for the labeling of 400-800 grids

Features

• For single and multiple labeling

• Gold particle sizes of 6, 10 ,15 and 25 nm

• Monodisperse particle population

• Minimal size variation and overlap

• OD520 nm of 1.0 to warrant cluster free storage

• Coefficient of variance: <12% for the 6 and 25 nm conjugates and <10% for the 10 and 15 nm conjugates

Application Instructions

On-Grid Labeling

For most applications grids are floated on top of drops of dilute reagent displayed on a sheet of Parafilm™.

The use of gold or nickel grids is recommended.

Whole mount and intact cell labeling

Specimens are kept floating in dilute reagent on a rocking table.

Recommended Incubation Solution:

PBS,

(10 mM Phosphate buffer, 150 mM NaCl),

0.1-0.2 % AURION BSA-C™

15 mM NaN3

pH 7.4

Specificity

Aurion offers the widest range of Conventional Immunogold Reagents. Please refer to the list of available

reagents under ‘Ordering Information’.

AURION Conventional Immunogold Reagents are prepared using the highest quality antibodies or binding

agents available. All antibodies are immuno affinity purified and immuno cross-adsorbed to reduce non-

specific reactions.

Information in the reactivity of Protein A and Protein G can be found in the section "Sharing our knowledge" or

by following this link.

Storage

AURION Conventional Immunogold Reagents have a guaranteed shelf life of 18 months from the date of

quality control analysis.

The products should be stored at 4-8°C. Freezing is not recommended.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Immunolabeling of the periplasmic space in ultrathin

cryosections of Escherichia coli with a protein A gold

conjugate.

* Courtesy M. de Jong

Instruction sheet (PDF)

Ordering informations

Anti-Rabbit Conventional

Catalog No.	Product Name	Size
806.011 / 106.011 810.011 / 110.011 815.011 / 115.011 825.011 / 125.011	Goat-anti-Rabbit IgG (H&L)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml
800.166 / 100.166 810.166 / 110.166 815.166 / 115.011 825.011 / 125.011	F(ab')2 fragment of Goat-anti-Rabbit IgG (H&L)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml
806.311 / 106.311 810.311 / 110.311 815.311 / 115.311 825.311 / 125.311	Donkey-anti-Rabbit IgG (H&L)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml

Anti-Mouse Conventional-1

Catalog No.	Product Name	Size
806.022 / 106.022 810.022 / 110.022 815.022 / 115.022 825.022 / 125.022	Goat-anti-Rabbit IgG (H&L)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml
806.033/ 106.033 810.033 / 110.033 815.033 / 115.033 825.033 / 125.033	Goat-anti-Mouse IgM (µ-chain)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml
806.044 / 106.044 810.044 / 110.044 815.044 / 115.044 825.044 / 125.044	Goat-anti Mouse IgG + IgM	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml

Anti-Mouse Conventional-2

Catalog No.	Product Name	Size
806.177 / 106.177 810.177 / 110.177 815.177 / 115.177 825.177 / 125.177	F(ab)'2 fragment of Goat-anti-Mouse IgG (H&L)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml
806.188 / 106.188 810.188 / 110.188 815.188 / 115.188 825.188 / 125.188	F(ab)'2 fragment of Goat-anti-Mouse IgG + IgM	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 ml
810.422 / 110.422	Goat-anti-Mouse IgG (H+L)xRat (cross adsorbed against Rat immunoglobulins)	10 nm, 1 ml / 2.5 ml
806.322 / 106.322 810.322 / 110.322 815.322 / 115.322 825.322 / 125.322	Dongkey-anti-Mouse IgG (H&L)	6 nm, 1 ml / 2.5 ml 10 nm, 1 ml / 2.5 ml 15 nm, 1.4 ml / 3.5 ml 25 nm, 1.4 ml / 3.5 mll

Anti-Rat Conventional

Catalog No.

Product Name

Size

806.055 / 106.055

810.055 / 110.055

815.055 / 115.055

825.055 / 125.055

Goat-anti-Rat IgG (H&L)

6 nm, 1 ml / 2.5 ml

10 nm, 1 ml / 2.5 ml

15 nm, 1.4 ml / 3.5 ml

25 nm, 1.4 ml / 3.5 ml

810.455 / 110.455

Goat-anti-Rat IgG (H+L)xMouse

10 nm, 1 ml / 2.5 ml

Anti-Human Conventional

Catalog No.

Product Name

Size

806.066 / 106.066

810.066 / 110.066

815.066 / 115.066

825.066 / 125.066

Goat-anti-Human IgG (H&L)

6 nm, 1 ml / 2.5 ml

10 nm, 1 ml / 2.5 ml

15 nm, 1.4 ml / 3.5 ml

25 nm, 1.4 ml / 3.5 ml

Anti-Goat Conventional

Catalog No.

Product Name

Size

806.077 / 106.077

810.077 / 110.077

815.077 / 115.077

825.077 / 125.077

Rabbit-anti-Goat IgG (H&L)

6 nm, 1 ml / 2.5 ml

10 nm, 1 ml / 2.5 ml

15 nm, 1.4 ml / 3.5 ml

25 nm, 1.4 ml / 3.5 ml

806.333 / 106.333

810.333 / 110.333

815.333 / 115.333

825.333 / 125.333

Donkey-anti-Goat IgG (H&L)

6 nm, 1 ml / 2.5 ml

10 nm, 1 ml / 2.5 ml

15 nm, 1.4 ml / 3.5 ml

25 nm, 1.4 ml / 3.5 ml

Anti-Biotin Conventional

Catalog No.

Product Name

Size

806.088 / 106.088

810.088 / 110.088

815.088 / 115.088

825.088 / 125.088

Goat-anti-biotin

6 nm, 1 ml / 2.5 ml

10 nm, 1 ml / 2.5 ml

15 nm, 1.4 ml / 3.5 ml

25 nm, 1.4 ml / 3.5 ml

Streptavidin Conventional

Catalog No.

Product Name

Size

806.099 / 106.099

810.099 / 110.099

815.099 / 115.099

825.099 / 125.099

Streptavidin Conventional

6 nm, 1 ml / 2.5 ml

10 nm, 1 ml / 2.5 ml

15 nm, 1.4 ml / 3.5 ml

25 nm, 1.4 ml / 3.5 ml

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