Products

ProceptorTM ELISA kits measure the CIC formed with

different immunoglobulin isotypes. The assay for

complement measure the complement bound directly

to CIC, thus providing the direct measurement of

complement activation. Figure No. 1 shows the

standard curve used for quantitating the complement

bound to CIC.

     Fig 1. Standard curves for the quantification of

     complement proteins C1q, C3, C4 and C5 bound

     to CIC.

Proceptor^TM ELISA targets only complement bound to CIC, thus measuring pathogenic CIC.    

Use Proceptor^TM assays to monitor complement activation from antibody based therapies.  

The complement regulatory proteins vitronectin and

clusterin are present two to three fold higher on

membrane attack complex (MAC) bound to CIC

(Figure No. 2). Company sells assays to measure

these proteins bound to CIC-MAC complex and to

soluble MAC (SMAC).

     Fig. 2 The amount of vitronectin and clusterin

     bound to soluble CIC in SLE patients

To define which complement pathway is contributing

in complement activation use the combination of the

assays from classical pathway C1q, C4, for

alternative pathway measure C3 and for terminal

pathway use either C5 or MAC.  

     Fig. 3 Complement proteins C1q, C3, C4 and C5

     present on CIC in SLE patients.

  Fig. 4 Effect of plasma pheresis on CIC and complement bound to CIC pre and post therapy.

Figure 4 demonstrate the changes on the levels of CIC composed of IgG isotype pre and post

plasmapheresis. The amount of C4 and C5 bound to CIC was also measured. Post plasmapheresis the

amount of the C4 and C5 dropped significantly. The drop was more then 80%, not accountable by decrease

in the levels of CIC.