Products

Proteins account for about 80% of keratinized animal tissues that include but not limited to hair, nail, horn and

wool. These tissues contain mainly hard α-keratins made of several distinctive types of protein with

molecular weight ranging from 10-135 Kda. Majority of the proteins are ranging from 10-65 Kda. Several

methods have been reported for protein extraction from keratinized animal tissues. The extraction buffer

usually contains high concentration of urea, thiourea, guanidinehydrochloride or a combination of these

strong denaturants. Protein extraction with high concentration denaturants is relatively effective but the

concentration of extracted protein is usually low due to larger extraction buffer/tissue ratio. Extracted proteins

need to be concentrated prior to further analysis. The presence of high concentration of denaturants may

also interfere with downstream application. HD-021 is designed to effectively extract proteins from keratinized

animal tissues without using strongdenaturants mentioned above. The extracted protein can be directly

loaded onto SDS-PAGE without concentration. The extraction buffer of this kit contains 0.5% SDS and other

chemicals. Extracted protein concentration is 1-2 mg/ml.