화학합성의 Chloride Channel Blocker Peptide Toxin(1)
본문
화학합성의 각종 Chloride Channel Blocker Peptide Toxin (1)
Chloride channel 연구에 유용한 유독 생물 유래의 합성 peptide toxin 입니다. 화학합성 peptide이기 때문에
endotoxin 오염의 걱정이 없습니다.
GaTx1
The GaTx1 peptide was originally isolated from the venom of Leiurus quinquestriatus hebraeus. This peptide is a potent
and selective inhibitor of CFTR, an anion selective channel belonging to the ABC transporter superfamily. It was shown to
block CFTR channels in a state dependent manner by acting from the internal face of the channel and it is supposed to
be a non competitive inhibitor of ATP-dependent channel gating. GaTx1 is a valuable tool to explore the function of
CFTR.
Details
|
AA sequence: |
|
Cys1-Gly-Pro-Cys4-Phe-Thr-Thr-Asp-His-Gln-Met-Glu-Gln-Lys-Cys15-Ala-Glu-Cys18-Cys19-Gly-
Gly-Ile-Gly-Lys-Cys25-Tyr-Gly-Pro-Gln-Cys30-Leu-Cys32-Asn-Arg-NH2 |
|
|
|
(Disulfide bonds between Cys1-Cys18, Cys4-Cys25, Cys15-Cys30 & Cys19-Cys32 ) |
|
Length (aa): |
|
34 |
|
Formula: |
|
C147H224N46O47S96 |
|
Molecular Weight: |
|
3674.2 Da |
|
Appearance: |
|
White lyophilized solid |
|
Solubility: |
|
Water and saline buffer |
|
CAS number: |
|
Not available |
|
Source: |
|
Synthetic |
References
1. Matthew D. Fuller, et al. (2007) State-dependent inhibition of cystic fibrosis transmembrane conductance regulator
2. Nael McCarty, et al. (2012) ABC transporter ligand GATX1. US patent 8,106,013
GaTx2
GaTx2 (gating modifier of anion channels 2) was isolated from the venom of Leiurus quinquestriatus hebraeus. GaTx2 is
the most potent peptide inhibitor of ClC-2 chloride channel ever described. Kd value is close to 20 pM. GaTx2 slows ClC-2
activation but without altering channel conductance. The effect is voltage-dependent. This inhibitory effect was
highlighted on rabbit ClC-2 channels expressed in oocytes. It has no effect on ClC-0, ClC-1, ClC-3, ClC-4, CFTR, GABAC,
Xenopus ClCa, Shaker B or Kv1.2 channels. Structurally, GaTx2 is composed of two β-strands and one α-helix. This
peptide is also called Leiuropeptide II. Bears 89, 93 and 96% identity with OdK1, neurotoxin PO1 and leiuropeptide III,
respectively.
Details
|
AA sequence: |
|
H-Val-Ser-Cys3-Glu-Asp-Cys6-Pro-Asp-His-Cys10-Ser-Thr-Gln-Lys-Ala-Arg-Ala-Lys-Cys19-Asp-
Asn-Asp-Lys-Cys24-Val-Cys26-Glu-Pro-Ile-OH |
|
|
|
(Disulfide bonds between Cys3-Cys19, Cys6-Cys24 & Cys10-Cys26 ) |
|
Length (aa): |
|
29 |
|
Formula: |
|
C125H199N39O47S6 |
|
Molecular Weight: |
|
3191.25 Da |
|
Appearance: |
|
White lyophilized solid |
|
Solubility: |
|
Water and saline buffer |
|
CAS number: |
|
Not available |
|
Source: |
|
Synthetic |
Reference
1. Thompson CH, et al. (2009) Isolation and characterization of a high affinity peptide inhibitor of ClC-2 chloride channels.
Chlorotoxin
Chlorotoxin is a neurotoxin that was originally isolated from the venom of Leiurus quinquestriatus. Chlorotoxin binds to Cl-
channels (small conductance epithelial chloride channels) in the brain and spinal cord and inhibits Cl- influx. Chlorotoxin
most probably acts as a specific blocker, although residues both inside and outside of the pore region of the Cl- channels
participate in chlorotoxin binding.
Details
|
AA sequence: |
|
H-Met-Cys2-Met-Pro-Cys5-Phe-Thr-Thr-Asp-His-Gln-Met-Ala-Arg-Lys-Cys16-Asp-Asp-Cys19-
Cys20-Gly-Gly-Lys-Gly-Arg-Gly-Lys-Cys28-Tyr-Gly-Pro-Gln-Cys33-Leu-Cys35-Arg-NH2 |
|
|
|
(Disulfide bonds between Cys2-Cys19, Cys5-Cys26, Cys16-Cys33 and Cys20-Cys35) |
|
Length (aa): |
|
36 |
|
Formula: |
|
C158H249N53O47S11 |
|
Molecular Weight: |
|
3995.8 Da |
|
Appearance: |
|
White lyophilized solid |
|
Solubility: |
|
Water and saline buffer |
|
CAS number: |
|
163515-35-3 |
|
Source: |
|
Synthetic |
|
Purity rate: |
|
> 97% |
References
1. Debin JA, et al. Purification and characterization of chlorotoxin, a chloride channel ligand from the venom of the scorpion.
Am. J. Physiol. PubMed link
2. Soroceanu L. Modulation of glioma cell migration and invasion using Cl(-) and K(+) ion channel blockers. J Neurosci.
3. Lyons SA. Chlorotoxin, a scorpion-derived peptide, specifically binds to gliomas and tumors of neuroectodermal origin.
Glia. PubMed link
Ordering informations
|
Catalog No. |
Product Name |
Size |
|
13GTX001 |
GaTx1 |
0.1mg, 0.5mg
& 1.0mg |
|
10GTX002 |
GaTx2 |
0.1mg, 0.5mg
& 1.0mg |
|
08CHL001 |
Chlorotoxin |
0.1mg, 0.1mg,
0.5mg & 1.0mg |
* 다른 사이즈나 bulk 공급도 가능하오니 별도로 문의하여 주십시오.
▣ 관련 페이지 ; Smartox Biotechnology
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