Products

PSVue^? Fluorescent Kits for Labeling Apoptotic Cells, Bacteria & Other Anionic Membranes

Phosphatidylserine(PS)  검출 probe입니다.

 

PSVue^?는 bis(zinc²⁺ dipicolylamine : Zn-DPA)를 포함하는 fluorescent probes 입니다. Zn-DPA는 anionic

phospholipids에 높은 친화성을 나타내는 것으로 알려져 있으며, 세포막의 phosphatidylserine(PS)에 결합합니다.

apoptotic cells [1], necrotic cells [2], Gram+ and Gram- bacteria [3-5], activated cells, tumor vascular

endothelial cells, viruses 등의 검출에 사용할 수 있습니다.

 

 

Features:

• Bind to a variety of cell types which have negatively charged phospholipids exposed on their membranes

  including apoptotic cells [1], necrotic cells [2], Gram+ and Gram- bacteria [3-5], activated cells, tumor

  vascular endothelial cells, viruses, etc.

• Available in a range of detection wavelengths from long-UV to near infrared.

• Suitable for in vitro and in vivo use.

• Suitable for high-throughput screening assays.

• Bind to the same PS site as annexin-V.

 

Advantages of PSVue^? over Fluorescent Annexin-V:

• Binding kinetics are fast; annexin-V binding is slow

• Binding is Ca²⁺ independent; means no artifacts due to activation of nonspecific membrane scramblases

  by Ca²⁺.

• Cheap compared to most annexin-V fluorescent analogs

• Apoptosis can be detected under a wide variety of conditions(e.g. in presence of 10% serum, temps from 4

  to 37℃).

• Can provide more intense labeling due to their much smaller size (i.e. >10 PSVue^TM molecules can bind

  to the same area as 1 annexin V molecule).

 

 

Fig. 1. General Structure of PSVue^? Probes

 

 

Fig. 2. Micrographs (60X magnification) of Jurkat cells, treated with cytotoxic camptothecin (10 μM) for 3.5 h

and stained simultaneously with Annexin VAlexa Fluor 488, and PSVue^? 794 (10 μM). Brightfield image of the

entire field of cells (A); cells stained with Annexin V-Alexa Fluor 488 (B); cells stained with PSVue^? 794 (C);

overlay of images A, B, and C (D). No staining of healthy cells was observed in the absence of

camptothecin. (Images courtesy of Dr. Bradley Smith of University of Notre Dame)

Brochure (PDF)

References:

• Ayesa U, Gray BD, Pak KY, Chong PL (2016) Liposomes Containing Lipid-Soluble Zn (II)-Bis-

  dipicolylamine Derivatives Show Potential to be targeted to Phosphatidylserine on the surface of Cancer

  cells. Mol. Pharmaceut. DOI: 10.1021/ acs.molpharmaceut.6b00760.

• Rice, D.R.; Clear, K.J.; Smith, B.D. 2016 Imaging and therapeutic applications of zinc(II)-dipicolylamine

  molecular probes for anionic biomembranes. Chem. Commun. 52, 8787-8801.

• Smith, B.D. 2015. Smart Molecules for Imaging, Sensing and Health (SMITH).Beilstein J. Org. Chem., 11,

  2540-2548.

• Chan MM, Gray BD, Pak KY, Fong DF. 2015. Non-invasive in vivo imaging of arthritis in a collagen-induced

  murine model with phosphatidylserine-binding near-infrared dye. Arthritis Res. Ther. doi:10.1186/s13075-

  015-0565-x .

• Li J, Gerlach RL, Jonsson CB, Gray BD, Pak KY, Ng CK. 2015. Characterization of 18F-dipicolylamine

  (DPA) derivatives in cells infected with influenza virus. Nucl Med Biol., 42, 283-291.

Catalog No.

Product Name

Description

P-1001

PSVue^? 794

 Product insert pdf

 The PSVue^?794 (formerly PSS-794) reagent kit contains

 components to provide ~ 0.68 mL of a 1 mM solution of PSVue794

 in aqueous solution. The compound exhibits absorbance and

 fluorescence excitation maximum at 794 nm and emission

 maximum at 810 nm. The labeling vehicle provided with the kit

 (Diluent X) is designed to maximize dye solubility and is suitable

 for in vitro and in vivo use.

P-1002

PSVue^? 380

 Product insert pdf

 The PSVue^?380 (formerly PSS-380) reagent kit contains

 components to provide ~ 0.40 mL of a 2 mM solution of

 PSVue^? 380 in 50% aqueous ethanol solution. The compound

 has an absorbance max at 380nm and a fluorescence emission

 max at 440nm.

P-1003

PSVue^? 480

 Product insert pdf

 The PSVue 480 (formerly PSS-480) reagent kit contains

 components to provide ~0.5 mL of a 1 mM solution of PSVue 480.

 The compound has an absorbance max at 480 nm and an

 fluorescence emission max at 519 nm

P-1004

PSVue^? Biotin

 Product insert pdf

 Vial contains 1mg of solid. PSVue^? biotin can be complexed with

 streptavidin-coated quantum dots (not provided) for in vivo and in

 vitro use. Procedures to formulate PSVue^? biotin and prepare the

 PSVue^? biotin-streptavidin -coated quantum dot complex are

 provided.

P-1005

PSVue^? 550

 Product insert pdf

 The PSVue^?550 reagent kit contains components to provide ~0.5

 mL of a 1 mM solution of PSVue^? 550. The compound has an

 absorbance max at 553 nm and an fluorescence emission max at

 615 nm

P-1006

PSVue^? 643

 Product insert pdf

 The PSVue643 kit contains 0.25mL of a 1mM solution of PSVue643

 in water. The compound has an absorbance max at 643nm and a

 fluorescence emission max at 658nm

P-1007

PSVue^? 794-control

 Product insert pdf

 This probe contains the same fluorophore present in PSVue 794

 but without the Zn-DPA targeting moiety attached.The kit contains

 0.6mL of a 1 mM solution of 794-control probe in aqueous

 solution.

 The compound exhibits absorbance and fluorescence excitation

 maximum at 787 nm and emission maximum at 808 nm

P-1009

PSVue^? 499-WS

(mSEEK) Optical

Probe

 PSVue499-WS, also known as mSEEK, is a water soluble

 microbial targeted fluorescent imaging agent comprising a Zn-

 DPA unit and a BODIPY dye

P-1010

Propidium Iodide

 Propidium Iodide 2mL: (1mg/mL in H2O)