Products

Permanent Linkers, Photoactivatable Linkers

Irradiation of diazirines with UV light (ca. 350-360 nm) yields a highly reactive carbene species that can

undergo insertions into C–C, C–H, O–H, and X–H (X = heteroatom) bonds of neighboring molecules to

irreversibly form a covalent bond (Fig. 1). The diazirine moiety is the smallest of all photophores, so

introduction of a diazirine-bearing amino acid into a peptide or protein usually does not impair its biological

activity. Further advantages of diazirine crosslinkers are their stability at room temperature and their relative

stability against nucleophiles as well as towards both acidic and basic conditions.

Fig. 1: Use of photo-phenylalanine for the identification of angiotensin-II receptor binding sites; ^125I is used

as a radiotracer.

Photo-Ethylamine*HCl

Photo-Benzoic acid  

Photo-Benzylamine*HCl

ATFB