Products

The proteasome is a protein degradation system that requires metabolic energy and polymerization of

ubiquitin that is different from lysosome-based protein degradation. The 26S proteasome is made up of two

subcomplexes (a 20S proteasome and one or two19S regulatory particles). The proteasomes have been

found mainly localized in cytosol but also detected in nucleus. Traditionally, ultracentrifugation and affinity

isolation are most common methods for isolation of proteasomes. These methods,though relatively effective,

are usually time consuming with low yield. Many affinity-based methods require harsh elution conditions that

may affect the activity of isolated proteasomes and limit certain downstream applications. In most cases, the

methods can’t distinguish proteasomes derived from cytosol or nucleus. To overcome these shortcomings,

we have developed spin-column-based kits for enrichment of proteasome from nuclei (Cytosolic

Proteasome Enrichment Kit is also available under Cat # PT-040). Theprotocol is simple and rapid with high

yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins and is

useful for the studies of proteasome structure and function. The highly enriched proteasomes can also be

used as a starting material for affinity purifications.