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Quinolinic acid ELISA kit

Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid

Tryptophan to NAD+, a co-factor of many enzymatic reactions.

The Quinolinic Acid ELISA kit is optimized for the quantitation of Quinolinic Acid (QUIN or QA) within serum

and plasma samples. This easy-to-use competitive assay is well-suited for both small and large series of

samples. Working with a low sample volume of 25µL, this assay enables pre-clinical and clinical sample

testings.

Cross-validation of Quinolinic Acid ELISA and LC/MS data in human serum samples Human

serum samples (n=28) were processed for Quinolinic Acid measurement by mean of both QA ELISA kit and

liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS

are highly correlated (R Squared=0,9954).

Typical standard curve of Quinolinic acid ELISA Example of standard curve obtained with the QA

ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Quinolinic Acid levels.

Specifications

Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway Quinolinic acid

(QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to

NAD+, a co-factor of many enzymatic reactions. It has been described to share several neurotoxic functions

particularly through the activation of the N-Methyl-D-Aspartate receptor. Its production occurring in myeloid

cells (macrophages, microglia, etc.) is mainly driven by in ammatory stress and is therefore suspected to

play a key role in neuroin ammatory disorders such as Alzheimer and Parkinson diseases but also Multiple

Sclerosis, Amyotrophic Lateral Scle- rosis. Hence, while QA has been proposed as a biomarker in these

indications, it might certainly warrant further investigation to serve as a surro- gate in other “in ammation”

related diseases such as cancer, metabolic disorders, etc.

Instruction sheet (PDF)

References;

1. Fertan et al. Effects of the Novel IDO Inhibitor DWG-1036 on the Behavior of Male and Female 3xTg-AD Mice. Front Pharmacol. 2019 Sep;24

2. Pierozan et al. Synergistic Toxicity of the Neurometabolites Quinolinic Acid and Homocysteine in Cortical Neurons and Astrocytes: Implications in Alzheimer's Disease. Neurotox Res. 2018 Jul;34

3. Hernandez-Martinez et al. Quinolinic acid induces neuritogenesis in SH-SY5Y neuroblastoma cells independently of NMDA receptor activation. Eur J Neurosci. 2017 Mar;45

4. Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13

5. Guillemin GJ. Quinolinic acid, the inescapable neurotoxin. FEBS J. 2012 Apr;279