Products

Thymosin beta-4 sequester actin monomers and form 1:1 complexes

with G-actin, maintaining the pool of unpolymerized actin

It is supplied in a stable form as lyophilized protein, fully functional

after reconstitution.

Protein

Thymosin beta-4 (Synonyms: T beta 4, Fx, TB4X, PTMB4, TMSB4)

Origin

Recombinant, human recombinant - untagged.

Molecular mass

5kDa

Protein description

Thymosin is a hormone secreted from the thymus. The predominant form of

thymosin, thymosin b4, is a member of a highly conserved family of actin monomer

sequestering proteins. b-thymosins are the primary regulators of unpolymerized

actin, and are essential for maintaining the small cytoplasmic pool of free G-actin

monomers required for rapid filament elongation and allowing for the flux of

monomers betweenthe thymosin bound pool and F-actin.

Actin interaction

The predominant form of thymosin, thymosin b4, is a member of a highly conserved

family of actin monomer sequestering proteins. b-thymosins are the primary

regulators of unpolymerized actin, and are essential for maintaining the small

cytoplasmic pool of free G-actin monomers required for rapid filament elongation and

allowing for the flux of monomers between the thymosin bound pool and F-actin

Form

Lyophilized, ready-to-use

Quantity

2 x 50 µg & 1.0 mg

Buffer

5mM Tris pH 8.1, 0.1mM DTT and 0.2% sucrose, when reconstituted with 50 µl

ultrapure water to obtain a 1.0mg/ml solution.

Purity

>98% by RT-HPLC.

Purification notes

Thymosin beta-4 protein was expressed in E.coli and further purified by Affinity

Chromatography (polyproline column). The pure protein was yielded by gel

filtration.

Protein conc.

Determined by RT-HPLC.

Storage instructions

Thymosin beta-4 is stored at -70°C upon arrival will be stable in performance for at

least 6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.