Products

Talin isolated from smooth muscle has a molecular

mass ~270kD. Talin consists of a 220kD rod

containing actin, vinculin and integrin binding sites

and an N-terminal 47kD head region with a FERM

domain. The actin-binding multiligand protein Talin

interacts e.g. with phospholipids, signaling molecules,

and cytoskeletal components.

Talin is supplied as a readily soluble protein,

in a stable form as lyophilized protein, fully functional

after reconstitution.

Protein

Talin

Origin

Smooth muscle, turkey

Molecular mass

~270kDa

Protein description

Talin isolated from smooth muscle has a molecular mass of ~270kD.

Talin consists of a 220kD rod containing actin, vinculin and integrin binding sites

and an N-terminal 47kD head region with a FERM domain. The actin-binding

multiligand protein Talin interacts e.g. with phospholipids, signaling molecules, and

cytoskeletal components.

Actin interaction

Actin binding protein

Form

Lyophilized, ready-to-use

Quantity

1 x 100 µg

Buffer

0.1M KCl, 10mM imidazole pH 7.4, 2mM MgCl2, 0.2mM DTT and 2% disaccharides,

when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

Purity

~94% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle.

Protein conc.

Determined by Biuret method.

Storage instructions

Upon reconstitution the product is stored on ice, This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze/ thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.