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Actin Binding Protein, Profilin
Profilin Interactions: G-actin, F-actin ...
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Profilin binds to actin monomers, the barbed end of actin filaments
and seems to play a role in mRNA splicing in the nucleus.
It is supplied as a highly purified and soluble protein, ready-to-use.
Profilin is supplied in a stable form as lyophilized protein,
fully functional after reconstitution.
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Profilin
Description
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Protein
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Proffilin (Synonyms: Profilin 1)
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Origin
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Human recombinant - untagged.
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Molecular mass
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5kDa
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Protein description
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Profilin (15kDa) regulates the G-actin pool by sequestration of actin monomers.
Thus profilin controls the growth of actin filaments, and is thus an essential
regulator of cellular locomotion and cell shape changes. Profilin is negatively
regulated by phospholipids (PIP2, InsP3), reversed by phospholipase C.
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Actin interaction
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Profilin binds to monomeric actin by occupying an actin-actin interaction site.
As a result, profilin sequesters actin from the G-actin pool. Profilin catalyzes the
exchange of actin-bound ADP to ATP. This converts less polymerizable ADP-actin
into fast polymerizable ATP-actin. Since profilin has a significantly higher affinity for
ATP-than for ADP-actin.
Another F-actin growth control by profiling is that profilin-actin
complexes are introduced into growing actin polymers by e.g. formin,
WASP and VASP.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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2 x 50 µg
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Buffer
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150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted
with 50ul ultrapure water to obtain a 1.0 mg/ml solution.
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Purity
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>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
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Purification notes
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Profilin protein was expressed in E. coli and further purified by affinity
Chromatography (Polyproline column). The pure protein was yielded by gel filtration.
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Protein conc.
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Determined by OD280 (0.1%=1.226)
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Storage instructions
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Profilin is stored at -70°C upon arrival will be stable in performance for at least
6 months from the date of purchase. The solubilized protein is kept on ice and
should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).
Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical applications.
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