Products

Actin Binding Protein, Gelsolin

High efficient molecular scissors.

Cytoplasmic and plasma-gelsolins are supplied as natively prepared, pure proteins. Gelsolins are readily soluble proteins, requiring Ca++ for activation. Gelsolins bind to the barbed ends of actin filaments, preventing exchange of monomers (capping).   Supplied in a stable form as lyophilized proteins, fully functional after reconstitution.

Gelsolin (plasma isoform)

Description

Properties

Gelsolin (cytoplasmic)

Description

Properties

Protein

TL40 (N-terminal cytoplasmic gelsolin)

Origin

Smooth muscle, porcine

Molecular mass

40kDa

Protein description

TL40 was obtained by thermolysin cleavage of smooth muscle gelsolin.

It consists of the N-terminal homologous subdomains S1-S3 (aa 1-361,

molecular mass: ~40kD) and is a Ca++- independent proteolytic fragment of native

gelsolin. TL40 is preferentially used in F-actin fragmentation assays in the presence

of EGTA.

Actin interaction

Ca++- insensitive F-actin severing.

Form

Lyophilized, ready-to-use

Quantity

1 x 50 µg

Buffer

Lyophilized TL40 (50µg) contains 20mM imidazole pH 7.2, 50mM KCl, 1.0mM EGTA

and 1.0mM DTT and 0.5% disaccharides, when reconstituted with 50µl ultrapure

water to obtain a 1.0mg/ml solution.

Purity & Activity

Purity>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Fragmentation activity: The fragmentation activity  of TL40 was determined by

Ostwald viscometry at 25°C. The specific viscosity of F-actin (1mg/ml) is reduced

by <45% after addition of TL40 at a molar ratio of 1:100 (TL40:A) within less than

2min. For this purpose the TL40 stock solution was used as described below

containing 1% disaccharides. F-actin (100mM KCl, 10mM Imidazole pH 7.4,

1mM ATP) was mixed with TL40 and instantly measured within a time course of

0-30min by ostwald viscometry.

Protein conc.

Determined by the Biuret method with actin (skeletal muscle, rabbit) as reference.

Storage instructions

Gelsolin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be used within 5 days. Storage in glycerol at -20°C results in a significant

decrase of the activity, and has to determined individually.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

Protein

Gelsolin (cytoplasmic)

Origin

Smooth muscle, porcine

Molecular mass

80kDa

Protein description

Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six

homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin

regulatory protein, possessing three different actin modulating activities: severing

of F-actin, nucleation of actin polymerization and capping of filaments barbed end.

Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and

two binding sites for PIP2.

Actin interaction

Ca++-sensitive actin capping, nucleating and severing protein. Kd actin-binding

= 50mM (barbed end).

Form

Lyophilized, ready-to-use

Quantity

1 x 250 µg, 4 x 250 µg,

Buffer

10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and

1% disaccharides, when reconstituted with 50µl ultrapure water to obtain a

1.0mg/ml solution.

Purity & Activity

Purity>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Fragmentation activity: The fragmentation activity of gelsolin was determined

by Ostwald viscometry at 25°C. The specific viscosity of F-actin

(1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100.

The gelsolin stock solution was used as described below containing

1% disaccharides. F-actin containing 100mM KCl, 10mM Imidazole pH 7.4,

1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition

of gelsolin.

Protein conc.

Determined by the Biuret method with actin (skeletal muscle, rabbit) as reference.

Storage instructions

Gelsolin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be used within 5 days. Storage in glycerol at -20°C results in a significant

decrase of the activity, and has to determined individually.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

Catalog No.

Product Name

Size

8324-01

Actin Binding Protein, Gelsolin (plasma isoform)

1 x 100 µg & 5 x 100 µg

8304-01

Actin Binding Protein, Gelsolin (cytoplasmic)

1 x 100 µg, 2 x 100 µg & 5 x 100 µg

8305-03

Actin Binding Protein, TL40 (N-terminal gelsolin)

1 x 100 µg & 5 x 100 µg

8304-02

Actin Binding Protein, Gelsolin (cytoplasmic)

1 x 250 ug & 4 x 250 ug