Products

Fascin (lat. for bundle ) is a monomeric protein that

bundles actin filaments with its two actin-binding

sites.

Protein

Fascin (Synonyms: Fascin 1, 55kDa actin-bundling protein, singed-like protein, p55)

Origin

human recombinant - untagged.

Molecular mass

55kDa

Protein description

Fascin is a monomeric actin bundling protein with a molecular mass of 55kDa.

Actin interaction

Ca⁺⁺-insensitive actin bundling protein.

Form

Lyophilized, ready-to-use

Quantity

50 µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

Purity & Activity

Purity:>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Bundling activity: 2 µM Atto565-Actin (Cat.#:8162-01) was incubated with Fascin

at a molar 1:10 and 1:20 to verify bundling by fluorecence microscopy.

All proteins were used as supplied, i.e. without dialysis. The actin stock

solution was pre-spun 15min, 15.000xg, 4°C.

Low speed cosedimentation assay was performed (20,000xg, 30min) with rabbit

skeletal muscle actin (Cat.#:8101-03) in the presence of sugars. At a molar ratio of

4.1:1 (actin:fascin), fascin was mixed with F-actin. After 30min at RT,

the samples were used for low speed spinning.

Purification notes

Fascin protein was amplified from human macrophage cDNA and expressed  as a

GST-tag fusion protein in E.coli. After purification by GST-Agarose and subsequent

GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.

Protein conc.

Determined by OD280 (0.1%=1.224).

Storage instructions

Fascin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Protein

Fascin (Synonyms: Fascin 1, 55kDa actin-bundling protein, singed-like protein, p55)

Origin

recombinant, human recombinant - untagged.

Molecular mass

55kDa

Protein description

Fascin is a monomeric actin bundling protein with a molecular mass of 55kDa.

Actin interaction

Ca⁺⁺-insensitive actin bundling protein.

Form

Lyophilized, ready-to-use

Quantity

1.0mg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted

with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.

Purity & Activity

Purity:>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Bundling activity: 2µM Atto565-Actin (Cat.#:8162-01) was incubated with Fascin

at a molar ratio of 1:10 and 1:20 to verify bundling by fluorecence microscopy.

All proteins were used as supplied, i.e. without dialysis. The actin stock

solution was pre-spun 15min, 15.000xg, 4°C.

Low speed cosedimentation assay was performed (20,000xg, 30min)

with rabbit skeletal muscle actin (Cat.#:8101-03) in the presence of sugars.

At a molar ratio of 4.1:1 (actin:fascin), fascin was mixed with F-actin.

After 30min at RT, the samples were used for low speed spinning.

Purification notes

Fascin protein was amplified from human as amacrophage cDNA and expressed

as a GST-tag fusion protein in E. coli. After purification by GST-Agarose

and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration

chromatography.

.

Protein conc.

Determined by OD280 (0.1%=1.224).

Storage instructions

Fascin is stored at -70°C upon arrival will be stable in performance for at least

6 months from the date of purchase. The solubilized protein is kept on ice and

should be stored at -20°C. in glycerol or cryoprotect (Cat.#: 6011-01).

Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.