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Actin Binding Protein, Cap Z
Actin dynamics under control.
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The capping proteins (CP) plays a major role in
regulating actin filament dynamics by blocking
addition and loss of actin monomers to the actin
filament.
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Actin Binding Protein
Description
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Protein
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Cap Z, Capping protein (non-muscle isoform)
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Origin
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recombinant, human recombinant
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Molecular mass
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66kDa
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Protein description
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The subunits of the heterodimeric capping protein CapZ possess a molecular mass
of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with
high affinity in a Ca++-independent manner to the barbed end of actin filaments
(1:1000 M/M).
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Actin interaction
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Ca++-insensitive barbed end capping protein. Kd actin binding =>1nM (barbed end).
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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50 µg
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Buffer
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150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted
with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.
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Purity & Activity
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Purity: >90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Capping activity: F-actin (cat.#.8101) containing 20% pyrene actin
(Cat.#:8112) was incubated with CapZ and diluted below the critical concentration.
Depolymerisation was followed by fluorometry and compared to an actin reference
without CapZ. Complete capping was obtained at a molar ratio of 1:1000
(CapZ:Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock
solutions were pre-spun 1h, 100.000xg, 4°C
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Purification notes
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The alpha-1 SU is untagged, while the beta-2 SU contains a His-tag. Using anti-His
antibodies, the protein can be identified by all suited immunological techniques.
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Protein conc.
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Determined by OD280 (0.1%=1.234).
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Storage instructions
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CapZ is stored at -70°C upon arrival will be stable in performance for at least
6 months from the date of purchase. The solubilized protein is kept on ice and
should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated
freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
Actin Binding Protein
Description
|
Protein
|
|
Cap Z, Capping protein (non-muscle isoform)
|
|
Origin
|
|
recombinant, human recombinant
|
|
Molecular mass
|
|
66kDa
|
|
Protein description
|
|
The subunits of the heterodimeric capping protein CapZ possess a molecular mass
of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with
high affinity in a Ca++-independent manner to the barbed end of actin filaments
(1:1000 M/M).
|
|
Actin interaction
|
|
Ca++-insensitive barbed end capping protein. Kd actin binding =>1nM (barbed end).
|
Properties
|
Form
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|
Lyophilized, ready-to-use
|
|
Quantity
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1.0mg
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|
Buffer
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|
150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted
with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.
|
|
Purity & Activity
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|
Purity: >90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Capping activity: F-actin (cat.#.8101) containing 20% pyrene actin
(Cat.#:8112) was incubated with CapZ and diluted below the critical concentration.
Depolymerisation was followed by fluorometry and compared to an actin reference
without CapZ. Complete capping was obtained at a molar ratio of 1:1000
(CapZ:Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock
solutions were pre-spun 1h, 100.000xg, 4°C
|
|
Purification notes
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|
The alpha-1 SU is untagged, while the beta-2 SU contains a His-tag. Using anti-His
antibodies, the protein can be identified by all suited immunological techniques.
|
|
Protein conc.
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|
Determined at OD280 (0.1%=1.234).
|
|
Storage instructions
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|
CapZ is stored at -70°C upon arrival will be stable in performance for at least
6 months from the date of purchase. The solubilized protein is kept on ice and
should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated
freeze / thaw cycles.
|
|
Shipping conditions
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|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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