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Pyrene Actin
Fast and Reliable Analyses of Actin Dynamics
Pyrene-actin conjugates are reliable tools for investigating actin dynamics.
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Pyrene actin is chemically modified G-actin
by covalent conjugation of a fluorescent
pyrenyl group to Cys 374 at the C-term of the
actin molecule.
Actin dynamics like polymerization, nucleation
etc. are routinely measured by fluorometry
using pyrene actin.
Hypermol guarantees a dye/protein molar
ratio of 1:1 (pyrene:actin) for pyrene actin
(100%) and a dye/protein molar ratio of 1:10
for pyrene actin (10%). Pyrene actin is highly
polymerizable and instantly soluble.
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Figure: Fluorometrical Analysis of Polymerization kinetics of skeletal muscle actin vs smooth muscle actin
and Depolymerization of skeletal muscle actin vs. smooth muscle actin.
Applications
Fluorometry / Pyrene Assays
Pyren Actin 10% (rabbit skeletal muscle alpha actin)
 Description
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Protein
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Pyrene Actin
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified
G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the
molecule.
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Conjugation ratio
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Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio
of 1.0
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1x0 mg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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99% by scanning densitometry.
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Purification notes
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Purified from bovine cardiac muscle, GPC.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Pyrene Actin stored at -70°C upon arrival will be stable in performance for at least
6 months from the date of purchase. Solubilized pyrene actin is kept on ice and
should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Pyren Actin 10% (alpha cardiac actin, bovine)
Description
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Protein
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Pyrene Actin
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Origin
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Alpha cardiac actin, bovine
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Molecular mass
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42kDa
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Protein description
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G-actin from cardiac muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified
G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the
molecule.
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Conjugation ratio
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Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio
of 1.0.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1.0 mg
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Buffer
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2mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry.
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Purification notes
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Purified from bovine cardiac muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Actin stored at -70°C upon arrival will be stable in performance for at least
6 months from the date of purchase. Solubilized pyrene actin is kept on ice and
should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Pyren Actin 100% (rabbit skeletal muscle alpha actin)
Description
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Protein
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Pyrene Actin
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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G-actin from cardiac muscle is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified
G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the
molecule.
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Conjugation ratio
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Pyrene Actin (100%) is 100% labelled and thus has a dye/protein molar ratio of 1.0.
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1.0 mg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry.
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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Pyrene actin is stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Solubilized pyrene-actin is kept on ice
and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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