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Biotin Actin
Biotin Actin-Conjugates
Ready-to-use, instant solubility.
Biotin-actin conjugates are used to investigate actin interactions in-vitro assays.
Biotin-actin from skeletal or cardiac muscle α-actin are prepared by labelling lysine residues of actin with
activated Biotin-esters. The advantage of this Biotin-actin is the non-cleavable 22 angstroms spacer arm,
making the protein readily available for detection.
Applications
Elisa asays, Motility assays, Microinjection, Pull-down assays
Biotin-Actin (alpha-actin, rabbit skeletal muscle)
Description
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Protein
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Biotin-Actin
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Protein description
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Biotin-Actin from rabbit skeletal muscle is chemically modified α-actin by
esterification of lysine residues with biotin.
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Conjugation ratio
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The degree of labelling (DOL) of Biotin-Actin is approx. 1:1 (biotin:actin)
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Properties
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Form
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Lyophilized, ready-to-use
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Quantity
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1x100 µg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and
0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a
1mg/ml solution.
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Purity
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>99% by scanning densitometry.
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein conc.
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Determined by the Biuret method
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Storage instructions
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Biotin-Actin is stored at -70°C upon arrival and will be stable in performance for
at least 6 months from the date of purchase. The solubilized protein is kept on ice
and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Biotin-Actin (alpha-cardiac actin, bovine cardiac muscle)
Description
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Protein
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Biotin-Actin
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Origin
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Cardiac muscle alpha actin, bovine
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Molecular mass
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43kDa
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Protein description
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Biotin-Actin from cardiac muscle is chemically modified α-actin by esterification
of lysine residues with biotin.
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Conjugation ratio
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The degree of labelling (DOL) of Biotin-Actin is approx. 0.5-1:1 (biotin:actin).
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Properties
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Form
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Lyophilized, ready-to-use
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|
Quantity
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1x100 µg
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|
Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purity
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>99% by scanning densitometry
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Purification notes
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Purified from bovine cardiac muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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|
Biotin-Actin is stored at -70°C upon arrival and will be stable in performance for
at least 6 months from the date of purchase. Solubilized protein is kept on
ice and should be used within 1 week. Avoid refreezing.
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|
Shipping conditions
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|
At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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