Products

Immobilized Ficin

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the

endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. Ficin is typically used to

cleave mouse IgG₁ as this are difficult to cleave with papain and pepsin. In the presence of 1mM or 10mM

cysteine, ficin generates F(ab′)₂ and Fab fragments respectively. The Fab and F(ab′)₂fragments can be

separated from whole IgG and Fc with either Immobilized Protein A or ion exchange chromatography. 

Immobilized Ficin is a convenient reagent for producing Fab and F(ab′)2 fragments as it avoids the need to

remove the ficin enzyme after digestion

Features

• Generate Fab and F(ab')₂ fragments

• For digestion of mouse IgG₁

• Eliminates contamination bu Ficin

Important Information

• Activity: 1-1.5mg ficin/ml of resin

• Support: 6% Cross-linked Agarose

Instruction sheet (pdf)

Catalog No.

Product Name

Size

786-793

Immobilized Ficin

5 ml Resin