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360-HCP(Host Cell Protein) ELISA 키트는 기존의 HCP 분석에서 문제가 되고 있던, 샘플 중 HCP가 완전히

검출되지 않은 단점을 보완한 최신의 제품입니다.

기존의 generic HCP 분석은 모든 과정을 단 한 종류의 항체로 하지만, "Enhanced generic 360-HCP ELISA"에서는

4 종류의 키트 (각각 다른 anti-HCP 항체를 사용)를 제공합니다. 여러 항체를 사용하여서 샘플에 가장 적합한

항체를 찾을 수 있습니다. 

 

            

   

The 360-HCP (Host Cell Protein) ELISA Approach

The enhanced generic 360-HCP approach produces host cell protein (HCP) assays that are demonstrably

superior to traditional generic HCP assays minimizing well-known limitations including the possibility that

anti-HCP antibodies might not comprehensively detect HCPs present in an end-user's sample. While

traditional generic HCP assays provide just a single antibody that must work for all processes the enhanced

generic 360-HCP ELISA provides four kits, each using a different anti-HCP antibody so you can try multiple

antibodies to find the one that works best for your samples. 

 

  

This unique approach increases the assay specificity and sensitivity and allows sponsors and biotech

companies to postpone the development of a cost-intensive specific HCP assay until a more informed

decision on the success of a biologic in development can be made.

  

How it works

 

     

  

How we made the antibodies

E.coli|360-HCP Assay is based on W3110 and BL21(DE3) cell lines which were fermented in different culture

media and conditions resulting in four antigen preparations with distinct HCP patterns. By immunizing goats

with these antigens, BioGenes has developed a panel of four different HCP ELISAs (type A to D) that together

build up the enhanced generic E.coli|360-HCP Assay. A high specificity for the antigen in all four assays

was determined by Coomassie staining of the E.coli HCP antigen and 2D western blotting with the respective

antibodies. 

 

Superior HCP Recovery

E.coli|360-HCP Assay has been widely tested on the basis of a great number of mock E.coli HCP samples.

In case of sample 1 a recovery of 80% was estimated using assay type B. For sample 2 and 3 the best

recovery rate was determined using kit type A. For sample 5 a sufficient recovery was only determined using

kit type D, recovery rates determined with the other three kit types were below 30%.

 

     

  

Product Specifications

Sensitivity E.coli | 360-HCP ELISA Kits (A–D)

 

     

 

• LOD between 0.2 – 0.5 ng/mL

• LOQ between 0.6 – 1.6 ng/mL

• Working range 2 – 100 ng/mL 

 

Instruction sheet (PDF)

Poster (PDF)