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Human Liver Stellate Cells (HLiStCs)

HLiStCs (cAP-0035) are initiated by hepatic artery perfusion digestion with ollagenase. HLiStCs are separated

from the mixture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. The

cells are shipped in proliferating culture with >90 confluence (the cells are provided @ passage 2) or in a

frozen vial. Stellate-Growth medium (contains 10% serum and growth supplements, Cat# cAP-31) is

recommended for cell culture and these cells have a minimum average populationdoubling capacity > 8

when cultured following the detailed protocol described below).

Characterization of the cells

Cytoplasmic alpha-SMA: >99% positive by immunofluorescence

Cytoplasmic PECAM1 < 1% positive by immunofluorescence

HLiStCs are negative for HIV-1, HBV, HCV, and mycoplasma.