Products

PURE-EVs(Extracellular Vesicles), Size Exclusion Chromatography columns

For isolating & purifying exosomes and extracellular vesicles (EVs)

Size Exclusion Chromatography (SEC) is considered one of the best methods for isolating and purifying

exosomes and extracellular vesicles (EVs) from different matrices. In particular, this technique is very

efficient for separating exosomes from the circulating proteins and does not affect the original shape and

functionality of the vesicles.

HBM has developed diff erent classes of SEC columns for EV purifi cation: PURE-EV and miniPURE-EV are

two diff erent size of columns for EV purifi cation from small volume (1.5 ml up to 100 μl) of different matrices.

In addition, HBM provides PURE-EV PLUS, which, together with the SEC column, includes MWCO (molecular

weight cut-off ) concentrators especially designed for concentrating the EV preparation. UsingPURE-EVs

PLUS you can get in approximately 15 minutes a concentrate EV preparation.

Features

• Easy and fast protocol (turnaround time approximately 15 minutes).

• Isolate exosome from small volumes of sample.

• PURE-EVs PLUS includes MWCO concentrators especially designed for EV concentration.

• Reusable up to 5 times.

• Easy to store and ship (4°C).

Applications

• Exosome isolation from biofl uids and cell media.

• Purification of pre-isolated EVs from contaminants

• Isolated exosomes are suitable for multiple analysis (NTA, ELISA, FACS, WB, EM, MS, nucleic acid

  extraction, etc).

PURE-EVs, SEC columns performance and applications

PURE-EVs, isolation of highly pure exosomes in approximately 15 minutes.

PURE-EVs column was rinsed with 1 ml of human plasma, 24 fractions (500 μl each one) have been

collected and analyzed by ELISA ExoTESTTM assay (see section 3, ExoTEST quantifi cation kit) and by

BCA test for determining respectively the exosome and total protein content. As shown in fi gure 1 and 2, EVs

are eluted in fractions 6 - 11 (turnaround time approximately 15 min), whereas the BCA analysis showed

thatthe peak of plasma proteins corresponded to the fractions 14 - 24.

The 24 fractions were collected in 4 groups (1-6; 7-12; 13-18; 19-24) and analyzed by NTA with Nanosight.

Figures 3, 4 and 5 show the correlations between the eluted fractions and the performed analysis: positive

matching between ExoTESTTM results and the particles analysis by NTA (fig 3), complete separation of EV

from the plasma circulating proteins (fig 4 and 5).

Fig. 1. Exosome isolation from human plasma by PURE-EVs columns.

Fig. 2. Matching of EV quantity and total protein content eluted in each single fraction. ExoTESTTM analysis

shows that EVs are eluted in fractions 6-11 and succesfully separated by the plasma circulating proteins

(eluted in fractions 14-24). ExoTEST results expressed in ratio-to-background.

Fig. 3. EV elution peak. ExoTESTTM vs NTA analysis

Fig. 4. EV elution vs circulating protein elution. NTA analysis compared to protein BCA test

Fig. 5. EV elution vs circulating protein elution. ExoTESTTM analysis compared to protein BCA test

Ordering informations

- PURE-EV, Size Excluson Chromatography columns

- miniPURE-EV, Size Exclusion Chromatography columns

- maxiPURE-EV, Size Exclusion Chromatography columns

- PURE-EV PLUS, Size Exclusion Chromatography column & TFF-Easy concentrator

- maxiPURE-EV PLUS, Size Exclusion Chromatography column & TFF-Easy concentrator

- PURE-EV complete kit, Size Exclusion Chromatography column & 2 TFF concentrators