Products

SARS-CoV-2 RNA Polymerase (RdRp) Assay for Drug Discovery    

The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome

coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is

a key drug target for this deadly virus. The SARS-CoV-2 RdRP assay is based on detection of the RNA

molecules synthesized by the RNA polymerase in the presence of an RNA template and NTPs. The assay

can be performed in a 384-well or 96-well plate format for tests of the enzyme activities of the virus RNA

polymerase (SARS-CoV-2 RdRp) and high throughput screening of inhibitors.   

The SARS-CoV-2 RNA polymerase assay kit-100 (Catalog No.S2RPA100K) includes 300 µl of 10 x Buffer,

50 µl of 50 x RNA template, 60 µl of 50 x  mM NTPs (ATP, UTP, GTP, CTP) and 700 µl of 10 x fluorescence

dye. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-

well plate format. The assay kit includes all reagents except the enzyme.

The SARS-CoV-2 RNA polymerase assay kit plus-20 (Catalog No.S2RPA020KE) includes 75 µl of 10 x Buffer,

10 µl of 50 x RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 150 µl of 10 x fluorescence dye and

10 µl of 50 x SARS-CoV-2 RdRp.  It is for 20 assays of SARS-CoV-2 RNA polymerase in a 384-well plate

format or 10 assays in a 96-well plate format.  

The SARS-CoV-2 RNA polymerase assay kit plus-100 (Catalog No.S2RPA100KE) includes 300 µl of 10 x

Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x  mM NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x

fluorescence dye and  50 µl of 50 x SARS-CoV-2 RdRp.  It is for 100 assays of SARS-CoV-2 RNA

polymerase in a 384-well plate format or 50 assays in a 96-well plate format.

Functional SARS-CoV-2 RdRp enzyme

The SARS-CoV-2 RdRp complex – for 100 assays (Catalog No. RDRP-100S2) includes 50 µl of 50 x RNA-

dependent RNA Polymerase composed of the complex of the nsp12, nsp7 and nsp8 proteins from SARS-

CoV-2 virus. It is for 100 assays in a 384-well plate assay format (25 µl reaction) or 50 assays in a 96-well

plate format (50 µl reaction).

Gel-based SARS-CoV-2 RdRp assay kit