ProCipitate, substitute to phenol / chloroform
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ProCipitate, substitute to phenol / chloroform
Protein removal/DNA enrichment & isolation reagent
무해한 단백질 제거 시약으로, 핵산 정제에 있어서 phenol/chloroform의 대체품으로 사용할 수 있습니다. 음전하를 띤 고분자 전해질을 기반으로 하고 있으며, 단백질 용액에 첨가하면 전하가 중화되고 단백질이 불용화됩니다. 핵산에 결합하지 않기 때문에 높은 회수율, 고 품질의 DNA 및 RNA를 정제할 수 있습니다.
Agarose gel electrophoresis and Southern blot analysis comparing genomic DNA from yeast purified by phenol/chloroform and by ProCipitate™.
A.
Lanes 1-4 are isolated by traditional phenol/ chloroform methods.
Lanes 5-8 were purified using non-hazardous ProCipitate™
Lanes 1 & 5 are undigested.
The other lanes were digested with restriction enzymes as follows:
(BAM HI – lanes 2 & 4),(Eco RI – lanes 3 & 7), (Hind III – lanes 4 & 8)
Lanes 1-4 are isolated by traditional phenol/ chloroform methods.
Lanes 5-8 were purified using non-hazardous ProCipitate™
Lanes 1 & 5 are undigested.
The other lanes were digested with restriction enzymes as follows:
(BAM HI – lanes 2 & 4),(Eco RI – lanes 3 & 7), (Hind III – lanes 4 & 8)
B.
Southern Blot. The DNA was transferred and hybridized to a labeled probe and exposed to film for 3 hours.
Lanes 1-8 correspond to A.
Southern Blot. The DNA was transferred and hybridized to a labeled probe and exposed to film for 3 hours.
Lanes 1-8 correspond to A.
ProCipitate™
- Non-hazardous substitute to phenol/chloroform
- Removes only the contaminants & leaves DNA alone
- Improves yield of DNA over alternative bind and elute systems
- Adaptable to any sample size, and can be automated
- Key component of the ProPrep™ line of application specific kits
ProCipitate™ is a unique protein removal reagent based upon patented elastomeric polyelectrolytes. The polymer chains of ProCipitate™ are initially extended in a high energy state due to an overall net
negative charge. When introduced to protein solutions, the charges are neutralized and the polymer chains collapse to a more favorable energy state; DNA and RNA remain unreacted. ProCipitate™ is
non-hazardous and can replace phenol/chloroform with the additional benefits of solid-phase suspensions: adaptability to filtration and automation. It is routinely used for plasmids, cosmids, BACs, and
genomic DNA, as well as RNA. ProCipitate™ can also be used to remove Proteinase K and other enzymes. ProCipitate™ provides high quality DNA suitable for automated sequencing, southern blotting,
and restriction digestion. ProCipitate™ is available as a suspension reagent and in ProPrep™ kits for specific applications and high-throughput 96 well filter formats.
Performance Characteristics
|
Protein |
ProCipitate : Sample |
Removal |
|
BSA, PBS @ 30 mg/ml |
1 : 1 |
> 99% |
|
BSA, 1%SDS @ 30 mg/ml |
1 : 1 |
> 99% |
|
BSA, 3M GuSCN @ 30 mg/ml |
1 : 1 |
> 95% |
|
Human Serum |
1 : 1 |
> 90% |
|
Human Serum, 1% Surfactant |
1 : 1 |
> 95% |
|
Nucleic Acid Recovery |
ProCipitate : Sample |
Removal |
|
Calf Thymus DNA, A260 = 1.00 |
1 : 1 |
> 95% |
|
Total RNA, A260 = 1.00 |
1 : 1 |
> 99% |
References ;
- U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.
- U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore.
- Transgenic labeling of hair cells in the zebrafish acousticolateralis system Brian M. McDermott Jr Gene Expression Patterns Volume 10, Issues 2-3, February-March 2010, Pages 113-118
- Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Ivan D. Kozekov, Robert J. Turesky, Guillermo R. Alas, Constance M. Harris, Thomas M. Harris, Carmelo J. Rizzo Chemical Research in Toxicology 2010 23 (11), 1701-1713
- Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblastsStephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman BMC Genomics. 2006; 7: 301. Published online 2006 November 29. doi: 10.1186/1471-2164-7-301.
- Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.
- Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.
Ordering informations ;
1. P0050-30 : ProCipitate™(30ml)
2. P0050-100 : ProCipitate™(100ml)
2. P0050-100 : ProCipitate™(100ml)
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