Albumin depletion kits, AlbuSorb / AlbuVoid
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Albumin depletion kits, AlbuSorb / AlbuVoid
Albusorb™
Albumin depletion kit from serum or plasma
다수의 전하를 갖는 elastomer의 표면에 단백질이 결합하는 성질을 이용하여 혈청이나 혈장에서 알부민을 제거하는 시약입니다. 시약을 추가하여 간단한 작업 만으로 정제가 완료됩니다. 또한 시료에
맞게 시약의 양을 조절 할 수 있기 때문에 경제적입니다.
• Removes >90% albumin from 30 mg albumin/ml sample
• Affinity-type equivalence, virtually no cross-reactivity with other proteins
• Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
• Economical new surface technology, not based on affinity chromatography
• Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
• The albumin depleted filtrate retains the enzymatic and biological activity
• Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf
• The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield of albumin depleted serum protein is 0.1-0.2 milligrams. Albusorb™ binds
albumin from serum or plasma and is compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS. Samples are
also prepared for biomarker discovery, toxicological studies for new drugs, enzyme assays, protein profiling using SELDI analysis and cytokines research.
AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low abundance proteins. AlbuSorb™ comes from a silica-based, separation
platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are
disposable eliminating cycle to cycle variance and cross-contamination.
Product Sheet (PDF)
AlbuVoid™
Albumin Depletion and Low Abundance Protein Enrichment Kit from Serum, Plasma, Tissues And Culture Media
혈청이나 혈장에서 알부민을 제거하는 kit 입니다. 혈청이나 혈장에서 알부민을 분리하여 미량 단백질과 저분자 단백질을 농축 할 수 있습니다. 키트에는 필요한 버퍼와 spin filter가 포함되어 있습니다.
• Albumin voids in flow-through >95%, with <30 minute bind/wash/elute protocol
• Low abundance enrichment equivalent or better than hexa-peptides or antibodies
• Disposable, cost-effective, no column regeneration or cross-contamination
• Mild elution maintains tertiary structure and simple transfer to secondary analysis
• The eluted fractions retain their enzymatic and biological activity
• Removes albumin from many species including human, mouse, sheep, bovine, goat, rat, calf, cell culture.
• The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
AlbuVoid™ is a albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low abundance, and/or low molecular weight proteins. The AlbuVoid™
protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. AlbuVoid™ considerably enhances resolution of proteins below
50 kD, a limitation of alternate enrichment protocols.
AlbuVoid™ does not bind albumin. All other proteins(except albumin) in the sample binds to AlbuVoid™ and then you can elute off all the proteins minus the albumin. Resulting in low
abundance serum protein enrichment. It is ideal for applications involving biomarker discovery, enzyme assays, toxicological studies for new drugs, protein profiling using SELDI analy
is, protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS and cytokines research.
AlbuVoid™ derives from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to facilitate weak binding of proteins, allowing for rapid elution from
the matrix without any foreknowledge of the variety of proteins contained in the starting sample. Because of its specific binding properties, AlbuVoid™ depletes high abundance proteins
in serum like albumin while improving the resolution of less abundant serum proteins.
Ordering informations
|
Catalog No. |
Product Name |
Size |
|
A185-6 |
Albusorb™ |
4.3ml of 25µL serum samples processed |
|
A185-18 |
13ml of 25µL serum samples processed | |
|
AVK-05 |
AlbuVoid™ |
5 preps, 200µL of samples can be processed per prep, |
|
AVK-10 |
10 preps, 200µL of samples can be processed per prep, | |
|
AVK-50 |
50 preps, 200µL of samples can be processed per prep, |
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