Minute™ Histone/DNA Binding Protein Extraction Kit
본문
Minute™ Histone/DNA Binding Protein Extraction Kit
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지 않습니다.
본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다.
Very simple steps
Histones and other DNA binding proteins play an important role in chromosome organization, gene regulation and posttranslational modification. Core components of histones can be covalently modified
through methylation, acetylation, phosphorylation, and sumoylation. These modifications are essential for gene expression, regulation, DNA repair and chromosome condensation. HP-014 is designed to
rapidly extract histones and otherDNA binding proteins from cultured cells and cells isolated from animal tissues.Unlike many other commercial histone extraction kits that employ acid or high salt
extraction methods, HP-014 extracts histone proteins through a completely different mechanism. This kit is equally efficient in extracting histones and other DNA binding proteins that can be used for
internal controls for histone modification analysis. In comparison acid extraction protocol selectively extracts basic proteins and a good internal control is usually not possible.
HP-014 can extract histones from 0.5 to 5 millioncells in less than 10 min with high protein yield (1-2.5 mg/ml) making it the fastest and most robust histone extraction kit available in the market.
Instruction sheet (PDF)
Ordering information
| Catalog No. | Product Name | Kit components | Amount |
| HP-014 | Minute™ Histone/DNA Binding Protein Extraction Kit | Buffer A | 25 ml |
| Buffer B | 25 ml | ||
| Filter cartridges | 50 | ||
| Collection tubes with cap | 50 |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
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