96-Well DNA Decatenation Assays
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96-Well DNA Decatenation Assays
High throughput DNA topo I, topo II and topo IV assays
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Human DNA topoisomerase II and bacterial DNA topoisomerase IV (the parC-parE complex) converts the concatenated DNA into decatenated DNA (decatenation reaction). Bacterial topoisomerase I and gyrases also have DNA decatenation activity. The 96-Well Topoisomerase DNA Decatenation Assays are in a 96-well assay plate format that can be used for high-throughput tests of human topo II and bacterial topo IV inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm).
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96-Well Topoisomerase DNA decatenation assay kit (enzyme not included)
Each 96-Well Topoisomerase DNA decatenation assay kit (Catalog No. TDD96K) includes the assay buffer, concatenated DNA, ATP, fluorescence dye, rinse buffer and a TDD filter plate for 96 assays of DNA decatenation reactions. The assay buffer is optimized for bacterial topoisomerase IV. DNA decatenation enzyme is not included.
96-Well Human Topo II DNA decatenation assay kit plus (enzyme included)
Each 96-Well Human Topo II DNA decatenation assay kit plus (Catalog No. HDD96KE) includes the assay buffer, concatenated DNA, human topo II alpha, ATP, fluorescence dye, rinse buffer and a TDD filter plate for 96 assays of human topo II DNA decatenation reactions.
96-Well E. coli Topo IV DNA decatenation assay kit plus (enzyme included)
Each 96-Well E. coli Topo IV DNA decatenation assay kit plus (Catalog No. EDD96KE) includes the assay buffer, concatenated DNA, E. coli topo IV, ATP, fluorescence dye, rinse buffer and a TDD filter plate for 96 assays of E. coli topo IV DNA decatenation reactions.
96-Well S. aureus Topo IV DNA decatenation assay kit plus (enzyme included)
Each 96-Well S. aureus Topo IV DNA Decatenation Assay Kit Plus (Catalog No. SDD96KE) includes the assay buffer, concatenated DNA, S. aureus topo IV, ATP, fluorescence dye, rinse buffer and a TDD filter plate for 96 assays of S. aureus topo IV DNA decatenation reactions.
96-Well E. coli Topo I DNA decatenation assay kit plus (enzyme included)
Each 96-Well E. coli Topo I DNA Decatenation Assay Kit Plus (Catalog No. T1DD-96KE) includes the assay buffer, concatenated DNA, E. coli topo I, fluorescence dye, rinse buffer and a TDD filter plate for 96 assays of E. coli topo I DNA decatenation reactions.
96-Well E. coli gyrase DNA decatenation assay kit plus (enzyme included)
Each 96-Well E. coli gyrase DNA Decatenation Assay Kit Plus (Catalog No. T2DD-96KE) includes the assay buffer, concatenated DNA, E. coli gyrase, ATP, fluorescence dye, rinse buffer and a TDD filter plate for 96 assays of E. coli gyrase DNA decatenation reactions.
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Ordering informations
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Catalog No.
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Product Name
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Size
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TDD96K
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96-Well Topoisomerase DNA Decatenation Assay Kit
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96 assays
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HDD96KE
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96-Well Human Topo II DNA decatenation assay kit plus
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96 assays
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EDD96KE
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96-Well E. coli Topo IV DNA decatenation assay kit plus
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96 assays
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SDD96KE
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96-Well S. aureus Topo IV DNA decatenation assay kit plus
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96 assays
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T1DD-96KE
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96-Well E. coli Topo I DNA decatenation assay kit plus
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96 assays
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T2DD-96KE
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96-Well E. coli gyrase DNA decatenation assay kit plus
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96 assays
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