Kinase & ATP binding proteins enrichment reagents
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KinaSorb™ Kinase (& ATP binding proteins) enrichment reagents
- Ratio of Total Kinase Activity* relative to total protein content increases 300% or more
- ATP binding protein recoverable, ~200 ug
- Up to 4X concentration of kinase volume, relative to starting volume
- 60 minute, scaleable protocol compatible with functional assays, electrophoresis and Mass Spec
- Phosphatase (neutral & alkaline) activity not detectable
- Improves protein normalization when comparing heterogeneous tissues
KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. In a patent pending process, ATP – the common substrate for kinases, is reversibly immobilized to metallic
oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts,
or approximately 2 mg total cellular
extract protein, but the process can be scaled up or down to accommodate different
sample volumes and protein concentrations. The methods are
reasonably specific to ATP; little Phosphodiesterase activity (an enzyme which binds to a structurally similar adenosine containing substrate - CAMP), was measured in the eluant after
KinaSorb™ treatment. The kit includes all necessary reagents for immediate use.
Ordering information
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Catalog No.
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Product Name
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Size
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KinaSorb™ Kinase
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10 (0.5 ml) & 50 (2.5 ml) preps
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▣ 관련 페이지 ; Biotech Support Group
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