Minute™ Single Cell Isolation Kit
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Minute™ Single Cell Isolation Kit
(For fresh and fixed tissues)
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Single cells isolated with this kit can be used as starting materials for chromosome immunoprecipitation (ChIP) and FACS
analysis. The single cell suspension can also be used as a starting material for isolation/purification of DNA, RNA, proteins
and other cellular components.
Very simple steps
Invent Biotechnologies Minute™ single cell isolation kit is composed of optimized tissue disaggregation buffers and specially
designed filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate single cells/nuclei from fresh/
frozen/formaldehyde fixed animal tissues. The tissue disaggregation buffers are formulated to gently disaggregate animal
tissues. The buffers donâ?™t contain any proteinasesthat may have an advert effect on cell surface marker detection. Due
to the use of filter cartridges with pre-defined pore size and thickness, single cell suspension can be isolated from fresh or
fixed tissues quickly with high yield and viability. Single cells isolated with the kit can be used for chromosome
immunoprecipitation (ChIP) and FACS analysis. The single cell suspension can also be used as a starting material for
isolation/purification of DNA, RNA, proteins and other cellular components.
Single Cell # Per 50 mg fresh mouse tissue and viability
| Tissues | Brain | Kidney | Liver | Lung | Spleen |
| Cell No. in Million | 4-5 | 5-6 | 5-6 | 6-7 | 15-16 |
| Viability(%) | 40-50 | 50-55 | 70-75 | 70-80 | 90-95 |
Single Cell# Per 50 mg formaldehyde fixed mouse tissue
| Tissues | Brain | Kidney | Liver | Lung | Spleen |
| Cell No. in Million | 4-5 | 4-5 | 4-5 | 5-6 | 13-15 |
Ordering information
| Catalog No. | Product Name | Kit components | Amount |
| SC-012 | Minute™ Single Cell Isolation Kit | Buffer A for non-fixed tissues (SC-012-N) | 25 ml |
| Buffer B for fixed tissues (SC-012-N) | 25 ml | ||
| Cell isolation filter cartridges(P-001) | 50 | ||
| Collection tubes with cap(P-002) | 50 | ||
| Plastic rods (P-003) | 4 |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
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