| Actin-Toolkit F-Actin Binding F-actin과 F-actin 결합 단백질의 결합을 coprecipitation experiment를 통해 확인 및 분석 할 수 있는 kit입니다.
Actin-Toolkit F-Actin Binding은 HYPERMOL사의 다양한 Actin-Toolkits 중의 하나로서, Actin-Toolkits는 actin의 polymerization, depolymerization에 영향을 미치는 actin 결합단백질이나 각종 화합물의 연구를 위해 개발된 제품입니다. Actin과의 결합실험에서 현미경관찰, 결정화실험 등 각각의 목적에 맞게 아래의 다른 제품들 중에서도 선택 할 수 있습니다. 모든 제품은 ready-to-use로 사용이 쉽고 조작도 간단합니다. Filaments, Bundles & Networks. The Actin-Toolkits F-Actin Binding is available with rabbit α skeletal muscle actin and bovine α cardiac actin. The kit is used to investigate interactions with actin, as well as actin filament bundling or network formation by ligands. The analysis of pharmacological compounds, proteins or lipids altering ligand-F-actin interaction, can be analyzed by user-specific methods (e.g. various types of spectroscopy, NMR, EPR) or by high speed centrifugation followed by SDS-PAGE analysis and/or immunoblot analysis. Applications - Proof of the biological activity of a ligand by actin binding
- Cosedimentation analysis of ligands binding to F-actin
- Testing of actin filament bundling activity or network formation by ligands
- Analysis of pharmacological compounds, proteins or lipids altering ligand-actin-interaction
α-actin from rabbit skeletal muscle Description | Protein | | Actin (α-Actin) | | Origin | | Skeletal muscle alpha actin, rabbit | | Molecular mass | | 42kDa | | Toolkit description | | Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Specific actin binding indicates that the ligand is correctly folded and structurally intact. The Actin-Toolkit F-Actin Binding is a well-established and commonly used method to characterize ligand-actin interaction: at high speed centrifugation ligands binding to individual actin filaments are co-sedimented. At low speed spinning a selective co-sedimentation of actin filament bundles or networks is achieved. Low speed assays instantly characterize ligands to induce actin suprastructures. The Actin-Toolkit F-Actin Binding was developed to characterize the actin-binding properties of ligands (native or recombinant proteins, recombinant fragments etc.) and support the detection even of cryptic actin-binding sites. Further suitable applications are in vitro compound analysis of the ligand-actin interactions by investigating effects of pharmacological compounds on ligand-actin complexes, as well as analysis introducing further ligands (proteins, lipids etc.) to ligand-actin complexes. The kit contains 4x1.0mg rabbit skeletal muscle actin (Cat. # 8010-03), buffers to keep actin functional and to prevent denaturing. A handbook introduces and guides through the experimental work with actin in F-actin Binding Assays. | | Purity | | >99% by scanning densitometry |
Properties
| Form | | Lyophilized, ready-to-use | | Kit content | | 4x1.0mg G-Actin (lyophilized powder) 1x50µg Filamin (lyophilized) 4x50ml MonoMix (lyophilized G-actin ATP-buffer) 1x1.0ml PolyMix, 10x stock (lyophilized F-actin ATP-buffer, pH 7.4) 1x1.0ml PolyMix 6, 10x stock (lyophilized F-actin ATP-buffer, pH 6.0) 1x1.0ml PolyMix 8, 10x stock (lyophilized F-actin ATP-buffer, pH 8.0) 1x0.5ml MgCl2 (solution) 1xToolkit Handbook F-Actin Binding | | Actin Buffer | | 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. | | Related products | | For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). | | Purification notes | | Purified from rabbit skeletal muscle, GPC. | | Protein concent. | | Determined by the Biuret method | | Storage instructions | | We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. | | Shipping conditions | | At ambient temperature. Upon delivery store at -70°C. | | Remarks | | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. | | CAS no. | | 51005-14-2 (Actin) |
α-actin from bovine cardiac muscle Description | Protein | | Actin (α-cardiac Actin) | | Origin | | Cardiac muscle alpha actin, bovine | | Molecular mass | | 42kDa | | Toolkit description | | Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Specific actin binding indicates that the ligand is correctly folded and structurally intact. The Actin-Toolkit F-Actin Binding is a well-established and commonly used method to characterize ligand-actin interaction: at high speed centrifugation ligands binding to individual actin filaments are co-sedimented. At low speed spinning a selective co-sedimentation of actin filament bundles or networks is achieved. Low speed assays instantly characterize ligands to induce actin suprastructures. The Actin-Toolkit F-Actin Binding was developed to characterize the actin-binding properties of ligands (native or recombinant proteins, recombinant fragments etc.) and support the detection even of cryptic actin-binding sites. Further suitable applications are in vitro compound analysis of the ligand-actin interactions by investigating effects of pharmacological compounds on ligand-actin complexes, as well as analysis introducing further ligands (proteins, lipids etc.) to ligand-actin complexes. The kit contains 4x1.0mg bovine cardiac muscle actin (Cat. # 8100-02), buffers to keep actin functional and to prevent denaturing. A handbook introduces and guides through the experimental work with actin in F-actin Binding Assays. | | Purity | | >99% by scanning densitometry |
Properties
| Form | | Lyophilized, ready-to-use | | Kit content | | 4x1.0mg Actin (lyophilized powder) 1x50µg Filamin (lyophilized) 4x50ml MonoMix (lyophilized G-actin ATP-buffer) 1x1.0ml PolyMix, 10x stock (lyophilized F-actin ATP-buffer, pH 7.4) 1x1.0ml PolyMix 6, 10x stock (lyophilized F-actin ATP-buffer, pH 6.0) 1x1.0ml PolyMix 8, 10x stock (lyophilized F-actin ATP-buffer, pH 8.0) 1x0.5ml MgCl2 (solution) 1xToolkit Handbook F-Actin Binding | | Actin Buffer | | 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. | | Related products | | For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). | | Purification notes | | Purified from rabbit skeletal muscle, GPC. | | Protein concent. | | Determined by the Biuret method | | Storage instructions | | We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. | | Shipping conditions | | At ambient temperature. Upon delivery store at -70°C. | | Remarks | | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Protein Sequence on NCBI
Actin-Tookit series |