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Zyagen offers high quality pure intact total RNA of normal human tissues that might difficult to obtain because of size,

anatomical complexity, or high RNase tissue content. Human tisuses were obtained from hospitals or certified tissue

banks in USA. Tissues are freshly harvested by surgeons during surgical operation or from donors donated their tissues

for scientific research.  Tissues specimens are procured under strict approved protocols with informed consent and the

utmost attention to issues of patient safety, anonymity and confidentiality.

Total RNA is routinely extracted from single healthy normal donor using the powerful classical guanidine isothiocyanate

-phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is

treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80^oC. The integrity of each

RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of

RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.

RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis,

cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction..

Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.

Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.

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