Eye Tissue Region Blot, Single Species
본문
Rat / Rabbit Eye Tissue Region Blot, Single Species
Rat 및 Rabbit의 조직에서 추출된 총 단백질을 미리 blot한 premade type의 membrane입니다. 총 단백질은 protease에 의한 단백질 분해를 최소화하기 위해 다양한 protease 억제제를 포함하는 변성 버퍼를 이용, 추출하여 4 ~ 20 % 변성 polyacrylamide gel로 전기영동 후, membrane에 blotting 한 것입니다. 각 lane에는 75μg의 단백질 이 로드되어 있어, 그대로 Westerblot 분석에 사용 할 수 있기 때문에 연구시간을 절약하는데 매우 효과적입니다.
The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat / rabbit eyes.
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Blot lane order 1. Protein marker 2. Rat / Rabbit Whole Eye Lysate 3. Rat / Rabbit Retina Lysate 4. Rat / Rabbit Cornea Lysate 5. Rat / Rabbit Aqueous Humor Lysate |
Ordering informations
Catalog No. |
Product Name |
Size |
TB44 |
Rat Eye Tissue Region Blot |
1 Blot |
TB45 |
Rabbit Eye Tissue Region Blot |
1 Blot |
▣ 관련 페이지 ; G-Biosciences
댓글목록
등록된 댓글이 없습니다.