Products

Mouse / Rat / Human Brain Tissue Region Blot, Single Species

Mouse, rat 및 human의 조직에서 추출된 총 단백질을 미리 blot한 premade type의 membrane입니다. 총 단백질

은 protease에 의한 단백질 분해를 최소화하기 위해 다양한 protease 억제제를 포함하는 변성 버퍼를 이용, 추출

하여 4 ~ 20 % 변성 polyacrylamide gel로 전기영동 후, membrane에 blotting 한 것입니다. 각 lane에는 75μg의

단백질이 로드되어 있어, 그대로 Westerblot 분석에 사용 할 수 있기 때문에 연구시간을 절약하는데 매우 효과적

입니다.

The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally

distinct regions of adult mouse / rat / human brains.

The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20%

denaturing polyacrylamide gel, along with a prestained molecular weight marker.  After the proteins are

resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.

The blots are ready to be blocked and probed with the antibodies of choice.

Note: We recommend that customers contact our technical department for the latest information on the blots

as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.

Blot lane order

 1. Protein marker

 2. Mouse / Rat / Human  Frontal Cortex Lysate

 3. Mouse / Rat / Human  Posterior Cortex Lysate

 4. Mouse / Rat / Human  Cerebellum Lysate

 5. Mouse / Rat / Human  Hippocampus Lysate

 6. Mouse / Rat / Human  Olfactory Bulb Lysate

 7. Mouse / Rat / Human  Striatum Lysate

 8. Mouse / Rat / Human  Thalamus Lysate

 9. Mouse / Rat / Human  Midbrain Lysate

10. Mouse / Rat  Entorhinal Cortex Lysate or Human Pons Lysate

11. Mouse / Rat  Pons Lysate or Human Medulla Lysate

12. Mouse / Rat  Medulla Lysate or Human Spinal Cord Lysate

13. Mouse / Rat  Human Spinal Cord or Human Temporal Lobe

14. Mouse / Rat / Human  Whole Brain Lysate

Catalog No.

Product Name

Size

TB40

Mouse Brain Tissue Region Blot

1 Blot

TB41

Rat Brain Tissue Region Blot

1 Blot

TB57

Human Brain Tissue Region Blot

1 Blot