Products

ProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated,

ready-to-use solution. The ProteaseArrest™ 100X solution format is suitable for small, analytical sample

applications, as >95% inhibition is achieved by adding 10µl ProteaseArrest™ per ml sample. For samples

with higher than normal protease levels, the volume of ProteaseArrest™ added can beincreasedfor greater

inhibition levels. Protease levels can easily be assayed with our Protease Assays.

 

The cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain and metallo-

proteases.

 

An optional EDTA solution is provided for enhanced metalloprotease inhibition. It is not present in the actual

ProteaseArrest™ cocktail as it would inhibit the activity of proteins that require divalent cations (Ca²⁺, Mg²⁺or

Mn²⁺) for their biological activity. In addition, EDTA will inhibit the purification of proteins using immobilized

metal affinity chromatography(IMAC).

 

Due to the optimized concentration of the various inhibitors, ProteaseArrest™ shows excellent inhibition of

protease activities and is therefore suitable for the protection of proteins during preparation of samples and

protein purification from animal tissues, plants, yeast and bacteria.

 

ProteaseArrest™ is also available as single use aliquots that are suitable for >95% protease inhibition in 10ml

solutions. These OneQuant™ ProteaseArrest™ are provided for additional protease inhibitor cocktail

convenience.   ProteaseArrest™ is also offered as a dry format, as ProteCEASE™, for those who prefer

reconstitutionprior to use.

 

ProteaseArrest™ Outperforms Tablet Cocktails

The ProteaseArrest™ format allows delivery of optimized concentrations of protease inhibitor, for example 2X

or higher concentrations can be added for tissues with higher than normal protease concentrations; a feature

not possible with tablet format protease inhibitor cocktails.

 

In our study, a 1X concentration of ProteaseArrest™ inhibits over 95% of protease activities (e.g. Mouse

Pancreas Extract: 0.5 milligrams per milliliter of protein). The ProteaseArrest™ protease inhibitor cocktails

demonstrate greater inhibition levels compared to similar protease inhibitor cocktails, including tablet formats.

 

Figure 1: ProteaseArrest™ has high, reproducible protease inhibition. Protease inhibition in mouse pancreas

lysate with proteaseArrest™ (EDTAfree) and a commercially available EDTA-free tablet protease cocktail

was compared, using Protease Screening Kit. The assay used 0.5mg/ml pancreas lysate and incubation

conditions of 37°C for 2.5 hours. ProteaseArrest™ inhibited over 95% of total proteases, almost 80%more

protease inhibition compared to the competitor's tablet protease inhibitor cocktail.

 

In independent studies, researchers have found that ProteaseArrest™ outperforms several leading

manufacturer's protease inhibitor cocktails, including tablet formats, in the purification of plant proteins. 

 

G-Biosciences Protease-Phosphatase Arrest provides full protection of protein samples from proteases and

phosphatases released during the preparation of cell and tissue lysates. Our Protease-PhosphataseArrest™

contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases

along with 5  phosphatase inhibitors that target all the phosphatase categories: serine/threonine(Ser/Thr)

specific, tyrosine specific and dual specificity phosphatases.

 

ProteaseArrest Family

 

 

References

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