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                  Very simple steps

Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs.The

methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable.

YD-016 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and

strong cell walls.These microbes include but not limited to yeast, filamentous fungus, gram positive and

negative bacteria, insect eggs and microalgae.This kit contains optimized detergent-free and EDTA-free

protein extraction buffers.The whole procedure takes less than 10 min to complete and the protein yield is in

the range of 2-4 mg/ml with un-biased protein representation.This kit features a single tube protocol and

works well for both log phase and stationary phase microbes. The materials provided are sufficient for 50

extractions.