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Actin-Toolkit TIRFM
High resolution imaging of actin interactions.
The complete solution for TIRF microscopy and other high resolution systems!
Actin-Toolkit TIRFM은 HYPERMOL사의 다양한 Actin-Toolkits 중의 하나로서, 표적 단백질이 actin에 미치는
영향을 TIRFM (Total Internal Reflection Fluorescence Microscopy)이나 기타 single-molecule imaging
techniques로 고해상도 분석을 위한 형광 actin 시료를 조제하는 kit입니다. 본 제품 외에도 다양한 actin 연구용
키트가 있습니다. 하단의 Actin toolkit series를 참조하시고, 모든 제품은 ready-to-use로 사용이 쉽고 조작도
간단합니다.
The Actin-Toolkit TIRFM is a complete ready-to-use kit for HR imaging techniques, but is very suited for
epifluorescence microscopy as well. The kits are used for applications ranging from single molecule
imaging to in-vitro motility assays.
Applications
- Analyses of actin ligand induced polymerization, depolymerization and nucleation
- Visualization of ligand-induced actin dynamics
- Microscopical analysis of actin filament bundles or networks
Atto488-Actin, rabbit skeletal muscle actin
Description
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Protein
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Atto488-Actin (α-Actin)
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Toolkit description
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The Actin-Toolkit TIRFM was developed to analyze actin dynamics by TIRF
microscopy or other fluorescence based single molecule imaging techniques.
The Toolkit contains all components required for TIRFM analyses and enables
the user to start TIRFM experiments straight ahead.
It includes Atto488-Actin (λex 488nm, λem 523nm) with a DOL of 0.3 as demanded
for TIRFM analysis. The Toolkit contains NEM-Myosin II as a tethering protein, and
all components required for flow chamber TIRF assays, including FlumaXx- an
efficient oxygen scavenger system - to protect actin filament from fragmentation
during analyses. A detailed description of the flow chamber assembly and TIRFM
actin polymerization assays is provided with the handbook.
In combination with the actin buffers contained in the kit, error-free handling is
guaranteed. The kit contains 5x100µg of α-skeletal muscle Atto488-Actin
conjugates and buffers to keep the actin functional.
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Purity
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>99% by scanning densitometry
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Properties
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Form
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Lyophilized and in solution (see kit content).
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Kit content
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5x100µg Atto488-Actin (lyophilized powder)
1x100µg NEM-Myosin II (lyophilized powder)
3x1.0ml passivation solution (5% BSA, fatty acid free)
1x1.0ml PolyMix (lyophilized F-actin ATP-buffer)
1x50ml MonoMix (lyophilized G-actin ATP-buffer)
1x10ml HSB 5x stock (solution,Myosin Buffer, pH 7.0)
1x0.5ml ME-Buffer (100x Mg-Exchange buffer)
1xToolkit Handbook TIRFM
10x Light protective tubes & tags
+ FluMaXx
Oxygen Scavenger System for single molecule imaging
(Cat. #: 5161-01)
Content
5x 250µl FluMaXx (2x stock, lyophilized)
Contains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2,
glucose, cryoprotectants (proprietary formula)
1x500µl ATP/DTT Mix (10x stock, lyophilized)
Contains: ATP, DTT
2x 1.5ml Methylcellulose stock solution (0.5% in milli-Q™ water)
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Actin Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
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Purification notes
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Purified from rabbit skeletal muscle, GPC.
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Protein concent.
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Determined by the Biuret method
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Storage instructions
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We guarantee stable performance of the kit components for 6 months when stored at
-70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1
week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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CAS no.
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51005-14-2 (Actin)
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Atto565-Actin, rabbit skeletal muscle actin
Description
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Protein
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Atto565-Actin (α-Actin)
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
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Toolkit description
|
|
The Actin-Toolkit TIRFM was developed to analyze actin dynamics by TIRF
microscopy or other fluorescence based single molecule imaging techniques.
The Toolkit contains all components required for TIRFM analyses and enables
the user to start TIRFM experiments straight ahead.
It includes Atto565-Actin (λex 563nm, λem 592nm) with a DOL of 0.3 as demanded
for TIRFM analysis. The Toolkit contains NEM-Myosin II as a tethering protein, and
all components required for flow chamber TIRF assays, including FlumaXx- an
efficient oxygen scavenger system - to protect actin filament from fragmentation
during analyses. A detailed description of the flow chamber assembly and TIRFM
actin polymerization assays is provided with the handbook.
In combination with the actin buffers contained in the kit, error-free handling is
guaranteed. The kit contains 5x100µg of α-skeletal muscle Atto565-Actin
conjugates and buffers to keep the actin functional.
|
|
Purity
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>99% by scanning densitometry
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Properties
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Form
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Lyophilized, ready-to-use
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|
Kit content
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5x100µg Atto565-Actin (lyophilized powder)
1x100µg NEM-Myosin II (lyophilized powder)
3x1.0ml passivation solution (5% BSA, fatty acid free)
1x1.0ml PolyMix (lyophilized F-actin ATP-buffer)
1x50ml MonoMix (lyophilized G-actin ATP-buffer)
1x10ml HSB 5x stock (solution,Myosin Buffer, pH 7.0)
1x0.5ml ME-Buffer (100x Mg-Exchange buffer)
1xToolkit Handbook TIRFM
10x Light protective tubes & tags
+ FluMaXx
Oxygen Scavenger System for single molecule imaging
(Cat. #: 5161-01)
Content
5x 250µl FluMaXx (2x stock, lyophilized)
Contains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2,
glucose, cryoprotectants (proprietary formula)
1x500µl ATP/DTT Mix (10x stock, lyophilized)
Contains: ATP, DTT
2x 1.5ml Methylcellulose stock solution (0.5% in milli-Q™ water)
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|
Actin Buffer
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|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purification notes
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|
Purified from rabbit skeletal muscle, GPC.
|
|
Protein concent.
|
|
Determined by the Biuret method
|
|
Storage instructions
|
|
We guarantee stable performance of the kit components for 6 months when stored at
-70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1
week. Avoid refreezing.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
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|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
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Atto647-Actin, rabbit skeletal muscle actin
Description
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Protein
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Atto647-Actin (α-Actin)
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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42kDa
|
|
Toolkit description
|
|
The Actin-Toolkit TIRFM was developed to analyze actin dynamics by TIRF
microscopy or other fluorescence based single molecule imaging techniques.
The Toolkit contains all components required for TIRFM analyses and enables
the user to start TIRFM experiments straight ahead.
It includes Atto647-Actin (λex 647nm, λem 668nm) with a DOL of 0.3 as demanded
for TIRFM analysis. The Toolkit contains NEM-Myosin II as a tethering protein, and
all components required for flow chamber TIRF assays, including FlumaXx- an
efficient oxygen scavenger system - to protect actin filament from fragmentation
during analyses. A detailed description of the flow chamber assembly and TIRFM
actin polymerization assays is provided with the handbook.
In combination with the actin buffers contained in the kit, error-free handling is
guaranteed. The kit contains 5x100µg of α-skeletal muscle Atto647-Actin
conjugates and buffers to keep the actin functional.
|
|
Purity
|
|
>99% by scanning densitometry
|
Properties
|
Form
|
|
Lyophilized and in solution (see kit content).
|
|
Kit content
|
|
5x100µg Atto647-Actin (lyophilized powder)
1x100µg NEM-Myosin II (lyophilized powder)
3x1.0ml passivation solution (5% BSA, fatty acid free)
1x1.0ml PolyMix (lyophilized F-actin ATP-buffer)
1x50ml MonoMix (lyophilized G-actin ATP-buffer)
1x10ml HSB 5x stock (solution,Myosin Buffer, pH 7.0)
1x0.5ml ME-Buffer (100x Mg-Exchange buffer)
1xToolkit Handbook TIRFM
10x Light protective tubes & tags
+ FluMaXx
Oxygen Scavenger System for single molecule imaging
(Cat. #: 5161-01)
Content
5x 250µl FluMaXx (2x stock, lyophilized)
Contains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2,
glucose, cryoprotectants (proprietary formula)
1x500µl ATP/DTT Mix (10x stock, lyophilized)
Contains: ATP, DTT
2x 1.5ml Methylcellulose stock solution (0.5% in milli-Q™ water)
|
|
Actin Buffer
|
|
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%
disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml
solution.
|
|
Purification notes
|
|
Purified from rabbit skeletal muscle, GPC.
|
|
Protein concent.
|
|
Determined by the Biuret method
|
|
Storage instructions
|
|
We guarantee stable performance of the kit components for 6 months when stored at
-70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1
week. Avoid refreezing.
|
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
|
CAS no.
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51005-14-2 (Actin)
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Protein Sequence on NCBI
Actin-Tookit series
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