Smooth Muscle Actin
Manufacturer Quality !
Our quality is your guarantee.
Actins from Hypermol are prepared to meet highest demands in research. We offer actins from skeletal,
cardiac, smooth and non-muscle sources and supply a first grade quality only. The favorite properties
of our Actin are characterized by the following properties:
• Highly pure;
• Fully polymerizable;
• Instant solubility;
• Ready-to-use;
• Batch quality-control tested;
Actins are supplied in a stable from as lyophilized proteins. They are ready-to-use and fully
functional after reconstitution.
Smooth muscle actin (from porcine smooth muscle)
Description
Protein
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Actin (smooth muscle actin)
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Origin
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Porcine smooth muscle.
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Molecular mass
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42kDa
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Toolkit description
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Smooth muscle actin (porcine) contains a mixture of ~80% gamma and ~20%
alpha smooth muslce actin. Actin is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. The typical polymerization and
depolymerization properties were compared with skeletal muscle actin in
fluorimetrical analyses (see product images). By mixing both actins with 10%
pyrene actin (rabbit skeletal muscle actin) different kineticsare observed.
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Purity
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>99% by scanning densitometry
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Properties
Form
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Lyophilized, ready-to-use
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Quantity
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1x1.0 mg
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Buffer
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2mM Tris-Cl pH 8.2, 1mM ATP, 0.5mM DTT, 0.1mM CaCl2 and 0.4% disaccharides,
when reconstituted with H2O.
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Purity
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Purity:>98% by scanning densitometry from Coomassie G-250 stained
SDS-Gels.
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Purification notes
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The native protein was purified from porcine smooth muscle.
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Protein conc.
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Determined by the Biuret method.
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Storage instructions
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The protein stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
Smooth muscle actin (from porcine smooth muscle)
Description
Protein
|
|
Actin (smooth muscle actin)
|
Origin
|
|
porcine smooth muscle.
|
Molecular mass
|
|
42kDa
|
Toolkit description
|
|
Smooth muscle actin (porcine) contains a mixture of ~80% gamma and ~20%
alpha smooth muslce actin. Actin is a single chain polypeptide with a molecular
weight of 42kD consisting of 375 amino acids. The typical polymerization and
depolymerization properties were compared with skeletal muscle actin in
fluorimetrical analyses (see product images). By mixing both actins with
10% pyrene actin (rabbit skeletal muscle actin) different kineticsare observed.
|
Purity
|
|
>99% by scanning densitometry
|
Properties
Form
|
|
Lyophilized, ready-to-use
|
Quantity
|
|
2 x 250 µg
|
Buffer
|
|
2mM Tris-Cl pH 8.2, 1mM ATP, 0.5mM DTT, 0.1mM CaCl2 and 0.4% disaccharides,
when reconstituted with H2O.
|
Purification notes
|
|
Purified from porcine brain, GPC.
|
Purity
|
|
Purity:>98% by scanning densitometry from Coomassie G-250 stained
SDS-Gels.
|
Protein conc.
|
|
Determined by the Biuret method.
|
Storage instructions
|
|
The protein stored at -70°C upon arrival will be stable in performance for
at least 6 months from the date of purchase. Avoid repeated freeze / thaw cycles.
|
Shipping conditions
|
|
At ambient temperature. Upon delivery store at -70°C.
|
Remarks
|
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or
Therapeutical Applications.
|
|