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MicroMolar Polyphosphate assay kit

시료 중에 미량 포함된 polyphosphate를 측정

Polyphosphate 농도를 키트에 포함된 형광색소와 polyphosphate가 결합하여 발생하는 형광을 이용하여 측정합니다.

PPK(polyphosphate kinase)에 대한 저해물질의 high throungput screeing에 유용하며, Inorganic phosphate,

pyrophosphate, ATP, ADP, AMP 등을 함유한 시료에 적용 할 수 있습니다.

Inorganic polyphosphate is a linear molecule composed of tens or hundreds of phosphate residues linked

together. In bacteria, polyphosphate kinase (PPK) converts polyphosphate and ADP to ATP. Lacking PPK activity

resulted in polyphosphate deficiency and failure in expression of RpoS (a sigma factor for RNA polymerase) that

leads to cell death. Thus PPK is a potential antibacterial drug target. Studies also suggestedthat large

accumulation of polyphosphate in cells responses to deficiencies in an amino acid, phosphate or nitrogen.

The MicroMolar Polyphosphate Assay Kit is for measurement of micromolar concentrations of polyphosphate. The

assay is based on increase of the fluorescence intensity (emission 550 nm, excitation 415 nm) of the kit

fluorescence dye PPD upon binding to polyphosphate.  The assay is compatible with regular buffers and various

phosphate compounds including inorganic phosphate, pyrophosphate, ATP, ADP and AMP. The assay kit can be

used for measurements of polyphosphate in biological samples or environmental water samples.  The kit can also

be used for biochemical assays of polyphosphate kinases in drug target characterization and high throughput

screen of inhibitors against polyphosphate kinases.

The kit provides the reagent for measurement of 1000 samples using 384-well plates (0.030 ml of sample volume)

or 500 samples using 96-well plates (0.060 ml of sample volume).  Cuvettes may also be used for measurements.

The kit also provides a polyphosphate, a 45-mer of sodium polyphosphate, as a control.

Catalog No.

Product Name

Size

 PPD1000

MicroMolar Polyphosphate assay kit

500 assays or 1000 assays